气候变化领域集成服务门户(CCPortal): COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions

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DOI	10.1073/pnas.2104847118
	COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions
	Urano E.; Okamura T.; Ono C.; Ueno S.; Nagata S.; Kamada H.; Higuchi M.; Furukawa M.; Kamitani W.; Matsuura Y.; Kawaoka Y.; Yasutomi Y.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:43
英文摘要	The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global threat to human health and life. A useful pathological animal model accurately reflecting human pathology is needed to overcome the COVID-19 crisis. In the present study, COVID-19 cynomolgus monkey models including monkeys with underlying diseases causing severe pathogenicity such as metabolic disease and elderly monkeys were examined. Cynomolgus macaques with various clinical conditions were intranasally and/or intratracheally inoculated with SARS-CoV-2. Infection with SARS-CoV-2 was found in mucosal swab samples, and a higher level and longer period of viral RNA was detected in elderly monkeys than in young monkeys. Pneumonia was confirmed in all of the monkeys by computed tomography images. When monkeys were readministrated SARS-CoV-2 at 56 d or later after initial infection all of the animals showed inflammatory responses without virus detection in swab samples. Surprisingly, in elderly monkeys reinfection showed transient severe pneumonia with increased levels of various serum cytokines and chemokines compared with those in primary infection. The results of this study indicated that the COVID-19 cynomolgus monkey model reflects the pathophysiology of humans and would be useful for elucidating the pathophysiology and developing therapeutic agents and vaccines. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	COVID-19; Elderly; Nonhuman primate; SARS-CoV-2; Underlying disease
语种	英语
scopus关键词	chemokine; cytokine; virus RNA; aging; animal experiment; animal model; animal tissue; Article; biological model; comparative study; computer assisted tomography; controlled study; coronavirus disease 2019; disease severity; female; human; human cell; inflammation; inoculation; Macaca fascicularis; male; metabolic disorder; nonhuman; oral swab; pathogenicity; pathology; pathophysiology; pneumonia; primary infection; protein blood level; reinfection; Severe acute respiratory syndrome coronavirus 2
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238772
作者单位	Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institutes of Biomedical Innovation, Health and Nutrition, Tsukuba, 305-0843, Japan; Laboratory of Virus Control, Research Institute for Microbial Diseases, Osaka University, Osaka, 565-0871, Japan; Department of Infectious Diseases and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, 371-8511, Japan; Laboratory of Antibody Design, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, 567-0085, Japan; Laboratory of Biopharmaceutical Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, 567-0085, Japan; Division of Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin–Madison, Madison, WI 53706, United States; Department of Special Pathogens, International Research ...
推荐引用方式 GB/T 7714	Urano E.,Okamura T.,Ono C.,et al. COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions[J],2021,118(43).
APA	Urano E..,Okamura T..,Ono C..,Ueno S..,Nagata S..,...&Yasutomi Y..(2021).COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions.Proceedings of the National Academy of Sciences of the United States of America,118(43).
MLA	Urano E.,et al."COVID-19 cynomolgus macaque model reflecting human COVID-19 pathological conditions".Proceedings of the National Academy of Sciences of the United States of America 118.43(2021).