气候变化领域集成服务门户(CCPortal): Assisted gene flow using cryopreserved sperm in critically endangered coral

CCPortal

DOI	10.1073/pnas.2110559118
	Assisted gene flow using cryopreserved sperm in critically endangered coral
	Hagedorn M.; Page C.A.; O'Neil K.L.; Flores D.M.; Tichy L.; Conn T.; Chamberland V.F.; Lager C.; Zuchowicz N.; Lohr K.; Blackburn H.; Vardi T.; Moore J.; Moore T.; Baums I.B.; Vermeij M.J.A.; Marhaver K.L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:38
英文摘要	Assisted gene flow (AGF) is a conservation intervention to accelerate species adaptation to climate change by importing genetic diversity into at-risk populations. Corals exemplify both the need for AGF and its technical challenges; corals have declined in abundance, suffered pervasive reproductive failures, and struggled to adapt to climate change, yet mature corals cannot be easily moved for breeding, and coral gametes lose viability within hours. Here, we report the successful demonstration of AGF in corals using cryopreserved sperm that was frozen for 2 to 10 y. We fertilized Acropora palmata eggs from the western Caribbean (Curaçao) with cryopreserved sperm from genetically distinct populations in the eastern and central Caribbean (Florida and Puerto Rico, respectively). We then confirmed interpopulation parentage in the Curaçao-Florida offspring using 19,696 single-nucleotide polymorphism markers. Thus, we provide evidence of reproductive compatibility of a Caribbean coral across a recognized barrier to gene flow. The 6-mo survival of AGF offspring was 42%, the highest ever achieved in this species, yielding the largest wildlife population ever raised from cryopreserved material. By breeding a critically endangered coral across its range without moving adults, we show that AGF using cryopreservation is a viable conservation tool to increase genetic diversity in threatened marine populations. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Acropora palmata; Assisted gene flow; Coral reproduction; Cryopreservation; Endangered species
语种	英语
scopus关键词	Acropora; adult; article; breeding; Caribbean; coral; cryopreservation; endangered species; Florida; gene flow; genetic association; genetic marker; genetic variability; human cell; male; nonhuman; parenthood; progeny; Puerto Rico; single nucleotide polymorphism; sperm; wildlife
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238643
作者单位	Center for Species Survival, Smithsonian Conservation Biology Institute, Front Royal, VA 22630, United States; Hawaii Institute of Marine Biology, Kaneohe, HI 96744, United States; Elizabeth Moore International Center for Coral Reef Research and Restoration, Mote Marine Laboratory, Summerland Key, FL 33042, United States; Center for Conservation, The Florida Aquarium, Apollo Beach, FL 33572, United States; Department of Biology, University of Bremen, Bremen, 28334, Germany; Department of Microbiology, Radboud University, Nijmegen, 6500, Netherlands; Department of Biology, The Pennsylvania State University, University Park, PA 16802, United States; Caribbean Research and Management of Biodiversity (CARMABI) Foundation, Piscaderabaai z/n Willemstad, Curaçao; SECORE International, Hilliard, OH 43026, United States; Institute for Biodiversity and Ecosystem Dynamics - Freshwater and Marine Ecology, University of Amsterdam, Amsterdam, 1098 XH, Netherlands; Program in Fisheries and Aquatic Sciences, School...
推荐引用方式 GB/T 7714	Hagedorn M.,Page C.A.,O'Neil K.L.,et al. Assisted gene flow using cryopreserved sperm in critically endangered coral[J],2021,118(38).
APA	Hagedorn M..,Page C.A..,O'Neil K.L..,Flores D.M..,Tichy L..,...&Marhaver K.L..(2021).Assisted gene flow using cryopreserved sperm in critically endangered coral.Proceedings of the National Academy of Sciences of the United States of America,118(38).
MLA	Hagedorn M.,et al."Assisted gene flow using cryopreserved sperm in critically endangered coral".Proceedings of the National Academy of Sciences of the United States of America 118.38(2021).