气候变化领域集成服务门户(CCPortal): The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily

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DOI	10.1073/pnas.2107335118
	The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily
	Zhao J.; Xie H.; Mehdipour A.R.; Safarian S.; Ermler U.; Münke C.; Thielmann Y.; Hummer G.; Ebersberger I.; Wang J.; Michel H.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:46
英文摘要	Multidrug and toxic compound extrusion (MATE) transporters are widespread in all domains of life. Bacterial MATE transporters confer multidrug resistance by utilizing an electrochemical gradient of H+ or Na+ to export xenobiotics across the membrane. Despite the availability of X-ray structures of several MATE transporters, a detailed understanding of the transport mechanism has remained elusive. Here we report the crystal structure of a MATE transporter from Aquifex aeolicus at 2.0-Å resolution. In light of its phylogenetic placement outside of the diversity of hitherto-described MATE transporters and the lack of conserved acidic residues, this protein may represent a subfamily of prokaryotic MATE transporters, which was proven by phylogenetic analysis. Furthermore, the crystal structure and substrate docking results indicate that the substrate binding site is located in the N bundle. The importance of residues surrounding this binding site was demonstrated by structure-based site-directed mutagenesis. We suggest that Aq_128 is functionally similar but structurally diverse from DinF subfamily transporters. Our results provide structural insights into the MATE transporter, which further advances our global understanding of this important transporter family. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Membrane protein structure; Multidrug resistance transporter; Phylogenetic analysis
语种	英语
scopus关键词	Aquifex aeolicus; article; binding site; crystal structure; membrane structure; multidrug resistance; nonhuman; phylogeny; site directed mutagenesis
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238599
作者单位	State Key Laboratory for Chemical Engineering, Collaborative Innovation Center of Chemical Science and Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Frankfurt am Main, 60438, Germany; Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Frankfurt am Main, 60438, Germany; Department for Applied Bioinformatics, Institute for Cell Biology and Neuroscience, Goethe University Frankfurt, Frankfurt am Main, 60438, Germany; Senckenberg Biodiversity and Climate Research Centre Frankfurt (BIK-F), Frankfurt am Main, 60325, Germany; LOEWE Centre for Translational Biodiversity Genomics, Frankfurt am Main, 60325, Germany
推荐引用方式 GB/T 7714	Zhao J.,Xie H.,Mehdipour A.R.,et al. The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily[J],2021,118(46).
APA	Zhao J..,Xie H..,Mehdipour A.R..,Safarian S..,Ermler U..,...&Michel H..(2021).The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily.Proceedings of the National Academy of Sciences of the United States of America,118(46).
MLA	Zhao J.,et al."The structure of the Aquifex aeolicus MATE family multidrug resistance transporter and sequence comparisons suggest the existence of a new subfamily".Proceedings of the National Academy of Sciences of the United States of America 118.46(2021).