气候变化领域集成服务门户(CCPortal): Reciprocal antagonistic regulation of E3 ligases controls ACC synthase stability and responses to stress

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DOI	10.1073/pnas.2011900118
	Reciprocal antagonistic regulation of E3 ligases controls ACC synthase stability and responses to stress
	Lee H.Y.; Park H.L.; Park C.; Chen Y.-C.; Yoon G.M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:34
英文摘要	Ethylene influences plant growth, development, and stress responses via crosstalk with other phytohormones; however, the underlying molecular mechanisms are still unclear. Here, we describe a mechanistic link between the brassinosteroid (BR) and ethylene biosynthesis, which regulates cellular protein homeostasis and stress responses. We demonstrate that as a scaffold, 1-aminocyclopropane-1-carboxylic acid (ACC) synthases (ACS), a rate-limiting enzyme in ethylene biosynthesis, promote the interaction between Seven-in-Absentia of Arabidopsis (SINAT), a RING-domain containing E3 ligase involved in stress response, and ETHYLENE OVERPRODUCER 1 (ETO1) and ETO1-like (EOL) proteins, the E3 ligase adaptors that target a subset of ACS isoforms. Each E3 ligase promotes the degradation of the other, and this reciprocally antagonistic interaction affects the protein stability of ACS. Furthermore, 14–3-3, a phosphoprotein-binding protein, interacts with SINAT in a BR-dependent manner, thus activating reciprocal degradation. Disrupted reciprocal degradation between the E3 ligases compromises the survival of plants in carbon-deficient conditions. Our study reveals a mechanism by which plants respond to stress by modulating the homeostasis of ACS and its cognate E3 ligases. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	ACC synthases; E3 ligases; Ethylene; Reciprocal degradation; SINAT
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238421
作者单位	Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47906, United States; The Center for Plant Biology, Purdue University, West Lafayette, IN 47906, United States
推荐引用方式 GB/T 7714	Lee H.Y.,Park H.L.,Park C.,et al. Reciprocal antagonistic regulation of E3 ligases controls ACC synthase stability and responses to stress[J],2021,118(34).
APA	Lee H.Y.,Park H.L.,Park C.,Chen Y.-C.,&Yoon G.M..(2021).Reciprocal antagonistic regulation of E3 ligases controls ACC synthase stability and responses to stress.Proceedings of the National Academy of Sciences of the United States of America,118(34).
MLA	Lee H.Y.,et al."Reciprocal antagonistic regulation of E3 ligases controls ACC synthase stability and responses to stress".Proceedings of the National Academy of Sciences of the United States of America 118.34(2021).