气候变化领域集成服务门户(CCPortal): Evidence for biosurfactant-induced flow in corners and bacterial spreading in unsaturated porous media

CCPortal

DOI	10.1073/pnas.2111060118
	Evidence for biosurfactant-induced flow in corners and bacterial spreading in unsaturated porous media
	Yang J.Q.; Sanfilippo J.E.; Abbasi N.; Gitai Z.; Bassler B.L.; Stone H.A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:38
英文摘要	The spread of pathogenic bacteria in unsaturated porous media, where air and liquid coexist in pore spaces, is the major cause of soil contamination by pathogens, soft rot in plants, food spoilage, and many pulmonary diseases. However, visualization and fundamental understanding of bacterial transport in unsaturated porous media are currently lacking, limiting the ability to address the above contamination- and disease-related issues. Here, we demonstrate a previously unreported mechanism by which bacterial cells are transported in unsaturated porous media. We discover that surfactant-producing bacteria can generate flows along corners through surfactant production that changes the wettability of the solid surface. The corner flow velocity is on the order of several millimeters per hour, which is the same order of magnitude as bacterial swarming, one of the fastest known modes of bacterial surface translocation. We successfully predict the critical corner angle for bacterial corner flow to occur based on the biosurfactant-induced change in the contact angle of the bacterial solution on the solid surface. Furthermore, we demonstrate that bacteria can indeed spread by producing biosurfactants in a model soil, which consists of packed angular grains. In addition, we demonstrate that bacterial corner flow is controlled by quorum sensing, the cell–cell communication process that regulates biosurfactant production. Understanding this previously unappreciated bacterial transport mechanism will enable more accurate predictions of bacterial spreading in soil and other unsaturated porous media. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacterial spreading; Biosurfactant; Corner flow; Unsaturated porous media; Wettability
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238376
作者单位	Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, United States; Saint Anthony Falls Laboratory, University of Minnesota, Minneapolis, MN 55414, United States; Department of Civil, Environmental, and Geo-Engineering, University of Minnesota, Minneapolis, MN 55455, United States; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States; Department of Biochemistry, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States; HHMI, Chevy Chase, MD 20815, United States
推荐引用方式 GB/T 7714	Yang J.Q.,Sanfilippo J.E.,Abbasi N.,et al. Evidence for biosurfactant-induced flow in corners and bacterial spreading in unsaturated porous media[J],2021,118(38).
APA	Yang J.Q.,Sanfilippo J.E.,Abbasi N.,Gitai Z.,Bassler B.L.,&Stone H.A..(2021).Evidence for biosurfactant-induced flow in corners and bacterial spreading in unsaturated porous media.Proceedings of the National Academy of Sciences of the United States of America,118(38).
MLA	Yang J.Q.,et al."Evidence for biosurfactant-induced flow in corners and bacterial spreading in unsaturated porous media".Proceedings of the National Academy of Sciences of the United States of America 118.38(2021).