气候变化领域集成服务门户(CCPortal): The translatome of neuronal cell bodies, dendrites, and axons

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DOI	10.1073/pnas.2113929118
	The translatome of neuronal cell bodies, dendrites, and axons
	Glock C.; Biever A.; Tushev G.; Nassim-Assir B.; Kao A.; Bartnik I.; Dieck S.T.; Schuman E.M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:43
英文摘要	To form synaptic connections and store information, neurons continuously remodel their proteomes. The impressive length of dendrites and axons imposes logistical challenges to maintain synaptic proteins at locations remote from the transcription source (the nucleus). The discovery of thousands of messenger RNAs (mRNAs) near synapses suggested that neurons overcome distance and gain autonomy by producing proteins locally. It is not generally known, however, if, how, and when localized mRNAs are translated into protein. To investigate the translational landscape in neuronal subregions, we performed simultaneous RNA sequencing (RNA-seq) and ribosome sequencing (Ribo-seq) from microdissected rodent brain slices to identify and quantify the transcriptome and translatome in cell bodies (somata) as well as dendrites and axons (neuropil). Thousands of transcripts were differentially translated between somatic and synaptic regions, with many scaffold and signaling molecules displaying increased translation levels in the neuropil. Most translational changes between compartments could be accounted for by differences in RNA abundance. Pervasive translational regulation was observed in both somata and neuropil influenced by specific mRNA features (e.g., untranslated region [UTR] length, RNA-binding protein [RBP] motifs, and upstream open reading frames [uORFs]). For over 800 mRNAs, the dominant source of translation was the neuropil. We constructed a searchable and interactive database for exploring mRNA transcripts and their translation levels in the somata and neuropil [MPI Brain Research, The mRNA translation landscape in the synaptic neuropil. https://public.brain.mpg.de/dashapps/localseq/. Accessed 5 October 2021]. Overall, our findings emphasize the substantial contribution of local translation to maintaining synaptic protein levels and indicate that on-site translational control is an important mechanism to control synaptic strength. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Dendrites; Local protein synthesis; RNA localization; Translatome
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238328
作者单位	Department of Synaptic Plasticity, Max Planck Institut fur Hirnforschung, Hessen, Frankfurt am Main, 60438, Germany
推荐引用方式 GB/T 7714	Glock C.,Biever A.,Tushev G.,et al. The translatome of neuronal cell bodies, dendrites, and axons[J],2021,118(43).
APA	Glock C..,Biever A..,Tushev G..,Nassim-Assir B..,Kao A..,...&Schuman E.M..(2021).The translatome of neuronal cell bodies, dendrites, and axons.Proceedings of the National Academy of Sciences of the United States of America,118(43).
MLA	Glock C.,et al."The translatome of neuronal cell bodies, dendrites, and axons".Proceedings of the National Academy of Sciences of the United States of America 118.43(2021).