气候变化领域集成服务门户(CCPortal): Relative abundance of prokaryotes in sediments of the Håkon Mosby mud volcano (Z1-Z3, all habitats)

CCPortal

DOI	https://doi.org/10.1594/PANGAEA.861872
	Relative abundance of prokaryotes in sediments of the Håkon Mosby mud volcano (Z1-Z3, all habitats)
	Ruff; S Emil; Ramette; Alban; Boetius; Antje
发布日期	2016-06-20
数据集类型	dataset
英文简介	CARD-FISH was performed as previously described in Ruff et al., (2013; doi:10.1371/journal.pone.0072627) with the following modifications. 4-6 µl of 25-fold diluted sediment were used for filtration. Archaeal cell walls were permeabilized with 0.1M HCl for 2 min to detect ANME-3 cells, or Proteinase K solution (15 µg ml-1 (Merck, Darmstadt, Germany) in 0.05 M EDTA (pH 8), 0.1 M Tris-HCl (pH 8), 0.5 M NaCl) for 2-4 min at room temperature for all other archaea. Bacterial cell walls were permeabilized with lysozyme solution (1000kU/ml) for 60 min at 37°. Cells were stained with DAPI (1µg/ml), embedded in mounting medium and counted in 40-60 independent microscopic fields using an Axiophot II epifluorescence microscope (Carl Zeiss, Jena, Germany).
空间范围	Median Latitude: 72.003918 * Median Longitude: 14.727823 * South-bound Latitude: 72.000300 * West-bound Longitude: 14.702167 * North-bound Latitude: 72.006660 * East-bound Longitude: 14.747660
时间范围	2003-07-02T00:00:00 - 2010-10-04T13:01:00
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/219317
推荐引用方式 GB/T 7714	Ruff,S Emil,Ramette,et al. Relative abundance of prokaryotes in sediments of the Håkon Mosby mud volcano (Z1-Z3, all habitats).2016-06-20.https://doi.org/10.1594/PANGAEA.861872.