气候变化领域集成服务门户(CCPortal): Hydrochemical and microbiological properties of groundwater from pristine aquifer sampled at the Lower Rhine Embayment

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DOI	https://doi.org/10.1594/PANGAEA.858491
	Hydrochemical and microbiological properties of groundwater from pristine aquifer sampled at the Lower Rhine Embayment
	Detmers; Jan; Strauss; H; Schulte; U; Bergmann; A; Knittel; Katrin; Kuever; Jan
发布日期	2016-06-19
数据集类型	dataset
英文简介	The hydrochemistry and the microbial diversity of a pristine aquifer system near Garzweiler, Germany next to the open-pit lignite mine Garzweiler 1, were characterized. Hydrogeochemical and isotopic data indicate a recent activity of sulfate-reducing bacteria in the Tertiary marine sands. The community structure in the aquifer was studied by fluorescence in situ hybridization (FISH). Up to 7.3 x 10**5 cells/ml were detected by DAPIstaining. Bacteria (identified by the probe EUB338) were dominant, representing 51.9% of the total cell number (DAPI). Another 25.7% of total cell were affiliated with the domain Archaea as identified by the probe ARCH915. Within the domain Bacteria, the beta-Proteobacteria were most abundant (21.0% of total cell counts). Using genusspecific probes for sulfate-reducing bacteria (SRB), 2.5% of the total cells were identified as members of the genus Desulfotomaculum. This reflects the predominant role these microorganisms have been found to play in sulfatereducing zones of aquifers at other sites. Previously, all SRB cultured from this site were from the spore-forming genera Desulfotomaculum and Desulfosporosinus. Samples were taken after pumping for >= 40 min and after parameters such as temperature, pH, redox potential, oxygen and conductivity of the groundwater had remained stable for >= 15 min due to recharge of aquifer water. Hybridization and microscopy counts of hybridized and 4',6'-diamidino-2-phenylindole (DAPI)- stained cells were performed as described in Snaidr et al., (1997, http://aem.asm.org/content/63/7/2884.full.pdf). Means were calculated from 10 to 20 randomly chosen fields on each filter section, corresponding to 800-1000 DAPI stained cells. Counting results were always corrected by subtracting signals observed with the probe NON338. Formamide concentrations and oligonucleotide probes used please see further details.
空间范围	Latitude: 51.128512 * Longitude: 6.849289
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/216893
推荐引用方式 GB/T 7714	Detmers,Jan,Strauss,et al. Hydrochemical and microbiological properties of groundwater from pristine aquifer sampled at the Lower Rhine Embayment.2016-06-19.https://doi.org/10.1594/PANGAEA.858491.