气候变化领域集成服务门户(CCPortal): (Supplement 1) Detailed information on environmental samples and sequence data

CCPortal

DOI	https://doi.org/10.1594/PANGAEA.868770
	(Supplement 1) Detailed information on environmental samples and sequence data
	Morard; Raphael; Lejzerowicz; Franck; Darling; Kate F; Lecroq-Bennet; Beatrice; Pedersen; Mikkel Winther; Orlando; Ludovic; Pawlowski; Jan; Mulitza; Stefan; De Vargas; Colomban; Kucera; Michal
发布日期	2016-11-23
数据集类型	dataset
英文简介	Deep-sea sediments constitute a unique archive of ocean change, fueled by a permanent rain of mineral and organic remains from the surface ocean. Until now, paleo-ecological analyses of this archive have been mostly based on information from taxa leaving fossils. In theory, environmental DNA (eDNA) in the sediment has the potential to provide information on non-fossilized taxa, allowing more comprehensive interpretations of the fossil record. Yet, the process controlling the transport and deposition of eDNA onto the sediment and the extent to which it preserves the features of past oceanic biota remains unknown. Planktonic foraminifera are the ideal taxa to allow an assessment of the eDNA signal modification during deposition because their fossils are well preserved in the sediment and their morphological taxonomy is documented by DNA barcodes. Specifically, we re-analyze foraminiferal-specific metabarcodes from 31 deep-sea sediment samples, which were shown to contain a small fraction of sequences from planktonic foraminifera. We confirm that the largest portion of the metabarcode originates from benthic bottom-dwelling foraminifera, representing the in situ community, but a small portion (<10 %) of the metabarcodes can be unambiguously assigned to planktonic taxa. These organisms live exclusively in the surface ocean and the recovered barcodes thus represent an allochthonous component deposited with the rain of organic remains from the surface ocean. We take advantage of the planktonic foraminifera portion of the metabarcodes to establish to what extent the structure of the surface ocean biota is preserved in sedimentary eDNA. We show that planktonic foraminifera DNA is preserved in a range of marine sediment types, the composition of the recovered eDNA metabarcode is replicable and that both the similarity structure and the diversity pattern are preserved. Our results suggest that sedimentary eDNA could preserve the ecological structure of the entire pelagic community, including non-fossilized taxa, thus opening new avenues for paleoceanographic and paleoecological studies.
空间范围	Median Latitude: 34.312035 * Median Longitude: 23.499871 * South-bound Latitude: -62.010800 * West-bound Longitude: -65.010000 * North-bound Latitude: 87.069600 * East-bound Longitude: 136.310000
时间范围	2007-03-22T00:00:00 - 2008-09-11T19:44:00
语种	英语
国家	国际
学科大类	气候变化
学科子类	气候变化
文献类型	数据集
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/216710
推荐引用方式 GB/T 7714	Morard,Raphael,Lejzerowicz,et al. (Supplement 1) Detailed information on environmental samples and sequence data.2016-11-23.https://doi.org/10.1594/PANGAEA.868770.