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DOI	10.1016/j.scib.2019.12.028
	Restriction of exogenous DNA expression by SAMHD1
	Gu C.; Ming L.; Fang Y.; Liu X.; Zhang J.; Zelinskyy G.; Deng Q.; Liu J.; Xie Y.
发表日期	2020
ISSN	20959273
起始页码	573
结束页码	586
卷号	65 期号:7
英文摘要	SAMHD1 (Sterile Alpha Motif and Histidine-aspartate Domain containing protein 1) has been documented as a host factor that restricts HIV-1 and some DNA viruses. In this work, we attempted to explore possible effects of SAMHD1 on exogenous DNA and show that SAMHD1 exerts a general inhibition on the expression of exogenous DNA in vitro and in mice. This inhibition is achieved through repressing transcription of exogenous DNA. Intriguingly, unlike SAMHD1’s restriction of HIV-1, such restriction does not require the dNTPase or RNase activities, or T592 phosphorylation of SAMHD1. Mechanistically, SAMHD1 enhances the expression of interferon regulatory factor-1 (IRF1), while IRF1 upregulation was demonstrated to inhibit exogenous DNA expression in a similar fashion as SAMHD1. IFNλ1, whose induction has been associated with IRF1 activation, is dispensable for SAMHD1/IRF1-mediated restriction of exogenous DNA, and neither type I nor II interferons appear to be involved. We also demonstrate that SAMHD1/IRF1-mediated restriction can effectively inhibit hepatitis B virus (HBV) antigen expression and progeny virus production in mouse models. In conclusion, these data support restriction of exogenous DNA as a novel function of SAMHD1. © 2020 Science China Press
关键词	Exogenous DNA Hepatitis B virus Interferon regulatory factor-1 SAMHD1
英文关键词	Amino acids; Antibiotics; Diseases; Glycoproteins; Mammals; Viruses; Antigen expression; DNA expressions; Domain-containing proteins; Hepatitis B virus; Interferon regulatory factor-1; Novel functions; SAMHD1; Virus productions; DNA
语种	英语
来源期刊	Science Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/207041
作者单位	Key Laboratory of Medical Molecular Virology (Ministry of Education/National Health Commission/Chinese Academy of Medical Sciences), Department of Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China; University Hospital Essen, Institute of Virology, Essen, 45147, Germany; Key Laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, China; Children's Hospital, Fudan University, Shanghai, 201102, China
推荐引用方式 GB/T 7714	Gu C.,Ming L.,Fang Y.,et al. Restriction of exogenous DNA expression by SAMHD1[J],2020,65(7).
APA	Gu C..,Ming L..,Fang Y..,Liu X..,Zhang J..,...&Xie Y..(2020).Restriction of exogenous DNA expression by SAMHD1.Science Bulletin,65(7).
MLA	Gu C.,et al."Restriction of exogenous DNA expression by SAMHD1".Science Bulletin 65.7(2020).