气候变化领域集成服务门户(CCPortal): Thermochemical wastewater valorization: Via enhanced microbial toxicity tolerance

CCPortal

DOI	10.1039/c8ee00460a
	Thermochemical wastewater valorization: Via enhanced microbial toxicity tolerance
	Jayakody L.N.; Johnson C.W.; Whitham J.M.; Giannone R.J.; Black B.A.; Cleveland N.S.; Klingeman D.M.; Michener W.E.; Olstad J.L.; Vardon D.R.; Brown R.C.; Brown S.D.; Hettich R.L.; Guss A.M.; Beckham G.T.
发表日期	2018
ISSN	17545692
起始页码	1625
结束页码	1638
卷号	11 期号:6
英文摘要	Thermochemical (TC) biomass conversion processes such as pyrolysis and liquefaction generate considerable amounts of wastewater, which often contains highly toxic compounds that are incredibly challenging to convert via standard wastewater treatment approaches such as anaerobic digestion. These streams represent a cost for TC biorefineries, and a potential valorization opportunity, if effective conversion methods are developed. The primary challenge hindering microbial conversion of TC wastewater is toxicity. In this study, we employ a robust bacterium, Pseudomonas putida, with TC wastewater streams to demonstrate that aldehydes are the most inhibitory compounds in these streams. Proteomics, transcriptomics, and fluorescence-based immunoassays of P. putida grown in a representative wastewater stream indicate that stress results from protein damage, which we hypothesize is a primary toxicity mechanism. Constitutive overexpression of the chaperone genes, groEL, groES, and clpB, in a genome-reduced P. putida strain improves the tolerance towards multiple TC wastewater samples up to 200-fold. Moreover, the concentration ranges of TC wastewater are industrially relevant for further bioprocess development for all wastewater streams examined here, representing different TC process configurations. Furthermore, we demonstrate proof-of-concept polyhydroxyalkanoate production from the usable carbon in an exemplary TC wastewater stream. Overall, this study demonstrates that protein quality control machinery and repair mechanisms can enable substantial gains in microbial tolerance to highly toxic substrates, including heterogeneous waste streams. When coupled to other metabolic engineering advances such as expanded substrate utilization and enhanced product accumulation, this study generally enables new strategies for biological conversion of highly-toxic, organic-rich wastewater via engineered aerobic monocultures or designer consortia. © 2018 The Royal Society of Chemistry.
英文关键词	Anaerobic digestion; Bacteria; Bioconversion; Machinery; Metabolic engineering; Molecular biology; Proteins; Substrates; Toxicity; Biomass conversion process; Bioprocess development; Microbial conversions; Poly-hydroxyalkanoate; Process configuration; Protein quality control; Substrate utilization; Wastewater valorization; Wastewater treatment; aldehyde; anaerobic digestion; bacterium; gene; inhibitor; liquefaction; quality control; thermochemistry; tolerance; toxicity; wastewater; wastewater treatment; Bacteria (microorganisms); Pseudomonas putida
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/190233
作者单位	National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, United States; Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN 37830, United States; Bioeconomy Institute and Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, United States; LanzaTech, Inc., Skokie, IL 60077, United States
推荐引用方式 GB/T 7714	Jayakody L.N.,Johnson C.W.,Whitham J.M.,et al. Thermochemical wastewater valorization: Via enhanced microbial toxicity tolerance[J],2018,11(6).
APA	Jayakody L.N..,Johnson C.W..,Whitham J.M..,Giannone R.J..,Black B.A..,...&Beckham G.T..(2018).Thermochemical wastewater valorization: Via enhanced microbial toxicity tolerance.Energy & Environmental Science,11(6).
MLA	Jayakody L.N.,et al."Thermochemical wastewater valorization: Via enhanced microbial toxicity tolerance".Energy & Environmental Science 11.6(2018).