气候变化领域集成服务门户(CCPortal): Biohybrid photoheterotrophic metabolism for significant enhancement of biological nitrogen fixation in pure microbial cultures

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DOI	10.1039/c9ee00705a
	Biohybrid photoheterotrophic metabolism for significant enhancement of biological nitrogen fixation in pure microbial cultures
	Wang B.; Xiao K.; Jiang Z.; Wang J.; Yu J.C.; Wong P.K.
发表日期	2019
ISSN	17545692
起始页码	2185
结束页码	2191
卷号	12 期号:7
英文摘要	We induce the coating of biocompatible cadmium sulfide (CdS) nanoparticles (NPs) on the living cell surface of a versatile photoheterotrophic bacterium, Rhodopseudomonas palustris. The photo-induced electrons from the CdS NPs significantly improve the biological nitrogen fixation in pure cultures of R. palustris, as shown by increased nitrogenase activity, additional H2 evolution, elevated reducing equivalents, and increased intracellular ammonia and l-amino acids. As a result, an additional 153% of solid biomass is accumulated by the biohybrid cells, with outstanding photosynthetic efficiency of 6.73% and a nearly unaffected malate usage efficiency of 0.06 g h-1. The number of NPs and the cross-membrane interface both play important roles in the efficient generation and transduction of electrons. The biohybrid cells continuously fix N2 when sufficient N is available, thus revealing excessive reducing power. The Calvin cycle also contributes 28.1% to the additional solid biomass in the presence of available CO2. The CdS-coated photoheterotrophic cells exhibit excellent practical feasibility with an industrial waste carbon source under a solar/dark cycle. This study provides a facile and expandable strategy for other studies of visible-light-driven ambient N2 fixation and advanced solar-to-chemical conversion. © The Royal Society of Chemistry 2019.
英文关键词	Ammonia; Biocompatibility; Cadmium sulfide; CdS nanoparticles; Cell membranes; Cytology; Efficiency; II-VI semiconductors; Image enhancement; Sulfur compounds; Biological nitrogen fixations; Chemical conversions; Microbial culture; Nitrogenase activity; Photoheterotrophic; Photosynthetic efficiency; Rhodopseudomonas palustris; Visible-light-driven; Nitrogen fixation; bacterium; bioactivity; biomass; cell component; enzyme; enzyme activity; industrial waste; metabolism; microbial activity; nitrogen fixation; Bacteria (microorganisms); Rhodopseudomonas palustris
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/189873
作者单位	Department of Chemistry, Chinese University of Hong Kong, Shatin, New Territories, 999077, Hong Kong; School of Life Sciences, Chinese University of Hong Kong, Shatin, New Territories, 999077, Hong Kong; Institute for Energy Research, Jiangsu University, Zhenjiang, 212013, China; School of Energy and Environment, City University of Hong Kong, Kowloon, 999077, Hong Kong; Department of Physics, Chinese University of Hong Kong, Shatin, New Territories, 999077, Hong Kong; Institute of Environmental Health and Pollution Control, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, 510006, China
推荐引用方式 GB/T 7714	Wang B.,Xiao K.,Jiang Z.,et al. Biohybrid photoheterotrophic metabolism for significant enhancement of biological nitrogen fixation in pure microbial cultures[J],2019,12(7).
APA	Wang B.,Xiao K.,Jiang Z.,Wang J.,Yu J.C.,&Wong P.K..(2019).Biohybrid photoheterotrophic metabolism for significant enhancement of biological nitrogen fixation in pure microbial cultures.Energy & Environmental Science,12(7).
MLA	Wang B.,et al."Biohybrid photoheterotrophic metabolism for significant enhancement of biological nitrogen fixation in pure microbial cultures".Energy & Environmental Science 12.7(2019).