气候变化领域集成服务门户(CCPortal): Rewiring photosynthesis: A photosystem I-hydrogenase chimera that makes H2: In vivo

CCPortal

DOI	10.1039/c9ee03859k
	Rewiring photosynthesis: A photosystem I-hydrogenase chimera that makes H2: In vivo
	Kanygin A.; Milrad Y.; Thummala C.; Reifschneider K.; Baker P.; Marco P.; Yacoby I.; Redding K.E.
发表日期	2020
ISSN	17545692
起始页码	2903
结束页码	2914
卷号	13 期号:9
英文摘要	Harnessing the power of photosynthesis to catalyze novel light-driven redox chemistry requires a way to intercept electron flow directly from the photosynthetic electron transport chain (PETC). As a proof of concept, an in vivo fusion of photosystem I (PSI) and algal hydrogenase was created by insertion of the HydA sequence into the PsaC subunit. The PSI and hydrogenase portions are co-assembled and active in vivo, effectively creating a new photosystem. Cells expressing only the PSI-hydrogenase chimera make hydrogen at high rates in a light-dependent fashion for several days. In these engineered cells, photosynthetic electron flow is directed away from CO2 fixation and towards proton reduction, demonstrating the possibility of driving novel redox chemistries using electrons from water splitting and the photosynthetic electron transport chain. This journal is © The Royal Society of Chemistry.
英文关键词	Photosynthesis; Electron flow; Intercept electrons; Photosynthetic electron transport; Photosystem i; Proof of concept; Proton reduction; Redox chemistry; Water splitting; Electron transport properties; carbon dioxide; carbon fixation; catalysis; catalyst; cell; gene expression; hydrogen; photosynthesis; redox conditions
语种	英语
来源期刊	Energy & Environmental Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/189571
作者单位	School of Molecular Sciences, Center for Bioenergy and Photosynthesis, Arizona State University, Tempe, AZ, United States; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel; Department of Environmental Science, Yogi Vemana University, Kadapa Andhra Pradesh, India
推荐引用方式 GB/T 7714	Kanygin A.,Milrad Y.,Thummala C.,et al. Rewiring photosynthesis: A photosystem I-hydrogenase chimera that makes H2: In vivo[J],2020,13(9).
APA	Kanygin A..,Milrad Y..,Thummala C..,Reifschneider K..,Baker P..,...&Redding K.E..(2020).Rewiring photosynthesis: A photosystem I-hydrogenase chimera that makes H2: In vivo.Energy & Environmental Science,13(9).
MLA	Kanygin A.,et al."Rewiring photosynthesis: A photosystem I-hydrogenase chimera that makes H2: In vivo".Energy & Environmental Science 13.9(2020).