气候变化领域集成服务门户(CCPortal): Endogenous membrane stress induces T6SS activity in Pseudomonas aeruginosa

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DOI	10.1073/pnas.2018365118
	Endogenous membrane stress induces T6SS activity in Pseudomonas aeruginosa
	Stolle A.-S.; Meader B.T.; Toska J.; Mekalanos J.J.
发表日期	2021
ISSN	00278424
卷号	118 期号:1
英文摘要	The type 6 secretion system (T6SS) is a dynamic organelle encoded by many gram-negative bacteria that can be used to kill competing bacterial prey species in densely occupied niches. Some predatory species, such as Vibrio cholerae, use their T6SS in an untargeted fashion while in contrast, Pseudomonas aeruginosa assembles and fires its T6SS apparatus only after detecting initial attacks by other bacterial prey cells; this targeted attack strategy has been termed the T6SS tit-for-tat response. Molecules that interact with the P. aeruginosa outer membrane such as polymyxin B can also trigger assembly of T6SS organelles via a signal transduction pathway that involves protein phosphorylation. Recent work suggests that a phospholipase T6SS effector (TseL) of V. cholerae can induce T6SS dynamic activity in P. aeruginosa when delivered to or expressed in the periplasmic space of this organism. Here, we report that inhibiting expression of essential genes involved in outer membrane biogenesis can also trigger T6SS activation in P. aeruginosa. Specifically, we developed a CRISPR interference (CRISPRi) system to knock down expression of bamA, tolB, and lptD and found that these knockdowns activated T6SS activity. This increase in T6SS activity was dependent on the same signal transduction pathway that was previously shown to be required for the tit-for-tat response. We conclude that outer membrane perturbation can be sensed by P. aeruginosa to activate the T6SS even when the disruption is generated by aberrant cell envelope biogenesis. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	CRISPRi; Essential gene; Membrane stress; T6SS activation
语种	英语
scopus关键词	polymyxin B; Article; bacterial cell; bacterial membrane; bacterial outer membrane; bacterial strain; cell organelle; clustered regularly interspaced short palindromic repeat; controlled study; flow cytometry; gene expression; gene silencing; nonhuman; priority journal; protein phosphorylation; Pseudomonas aeruginosa; RNA interference; RNA sequencing; signal transduction; type VI secretion system; Vibrio cholerae
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181141
作者单位	Department of Microbiology, Harvard Medical School, Boston, MA 02115, United States; Institute of Infectiology, Center for Molecular Biology of Inflammation, University of Münster, Münster, 48149, Germany
推荐引用方式 GB/T 7714	Stolle A.-S.,Meader B.T.,Toska J.,et al. Endogenous membrane stress induces T6SS activity in Pseudomonas aeruginosa[J],2021,118(1).
APA	Stolle A.-S.,Meader B.T.,Toska J.,&Mekalanos J.J..(2021).Endogenous membrane stress induces T6SS activity in Pseudomonas aeruginosa.Proceedings of the National Academy of Sciences of the United States of America,118(1).
MLA	Stolle A.-S.,et al."Endogenous membrane stress induces T6SS activity in Pseudomonas aeruginosa".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021).