气候变化领域集成服务门户(CCPortal): Deep learning for in vivo near-infrared imaging

CCPortal

DOI	10.1073/PNAS.2021446118
	Deep learning for in vivo near-infrared imaging
	Ma Z.; Wang F.; Wang W.; Zhong Y.; Dai H.
发表日期	2021
ISSN	00278424
卷号	118 期号:1
英文摘要	Detecting fluorescence in the second near-infrared window (NIR-II) up to ∼1,700 nm has emerged as a novel in vivo imaging modality with high spatial and temporal resolution through millimeter tissue depths. Imaging in the NIR-IIb window (1,500–1,700 nm) is the most effective one-photon approach to suppressing light scattering and maximizing imaging penetration depth, but relies on nanoparticle probes such as PbS/CdS containing toxic elements. On the other hand, imaging the NIR-I (700–1,000 nm) or NIR-IIa window (1,000–1,300 nm) can be done using biocompatible small-molecule fluorescent probes including US Food and Drug Administration-approved dyes such as indocyanine green (ICG), but has a caveat of suboptimal imaging quality due to light scattering. It is highly desired to achieve the performance of NIR-IIb imaging using molecular probes approved for human use. Here, we trained artificial neural networks to transform a fluorescence image in the shorter-wavelength NIR window of 900–1,300 nm (NIR-I/ IIa) to an image resembling an NIR-IIb image. With deep-learning translation, in vivo lymph node imaging with ICG achieved an unprecedented signal-to-background ratio of >100. Using preclinical fluorophores such as IRDye-800, translation of ∼900-nm NIR molecular imaging of PD-L1 or EGFR greatly enhanced tumor-to-normal tissue ratio up to ∼20 from ∼5 and improved tumor margin localization. Further, deep learning greatly improved in vivo noninvasive NIR-II light-sheet microscopy (LSM) in resolution and signal/background. NIR imaging equipped with deep learning could facilitate basic biomedical research and empower clinical diagnostics and imaging-guided surgery in the clinic. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Deep learning; Near-infrared imaging; Second near-infrared window
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181121
作者单位	Department of Chemistry, Bio-X Program, Stanford University, Stanford, CA 94305, United States
推荐引用方式 GB/T 7714	Ma Z.,Wang F.,Wang W.,et al. Deep learning for in vivo near-infrared imaging[J],2021,118(1).
APA	Ma Z.,Wang F.,Wang W.,Zhong Y.,&Dai H..(2021).Deep learning for in vivo near-infrared imaging.Proceedings of the National Academy of Sciences of the United States of America,118(1).
MLA	Ma Z.,et al."Deep learning for in vivo near-infrared imaging".Proceedings of the National Academy of Sciences of the United States of America 118.1(2021).