CCPortal
DOI10.1073/pnas.2005590118
Single-cell transcriptomic analysis reveals the immune landscape of lung in steroid-resistant asthma exacerbation
Wang L.; Netto K.G.; Zhou L.; Liu X.; Wang M.; Zhang G.; Foster P.S.; Li F.; Yang M.
发表日期2021
ISSN00278424
卷号118期号:2
英文摘要Exaggerated airway hyperresponsiveness and inflammation are hallmarks of asthma, and lipopolysaccharide (LPS) exposure is linked to the severity of the disease and steroid resistance. To investigate the mechanisms underlying asthma exacerbation, we established a mouse model of LPS-induced steroid-resistant exacerbation on the background of house dust mite (HDM)-induced asthma to profile the immune cells in lung by using single-cell RNA deep sequencing. Twenty immune subsets were identified by their molecular and functional properties. Specific cell clusters of basophils, type 2 innate lymphoid cells (ILC2), and CD8+ memory T cells were the predominant sources of interleukin (IL)-4 and IL-13 transcripts whose expressions were dexamethasone resistant. Production of IL-13 by these cells was validated by IL-13-reporter mice. Neutralization of IL-13 abolished HDM/LPS-induced airway hyperresponsiveness, airway inflammation, and decreased mucus hypersecretion. Furthermore, using Ingenuity Pathway Analysis systems, we identified canonical pathways and upstream regulators that regulate the activation of basophils, ILC2, and CD8+ memory T cells. Our study provides mechanistic insights and an important reference resource for further understanding of the immune landscape during asthma exacerbation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词Asthma exacerbation; Pathway analysis; Single-cell RNA sequencing
语种英语
来源期刊Proceedings of the National Academy of Sciences of the United States of America
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/181075
作者单位Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450052, China; Department of Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, 450052, China; Priority Research Centre for Healthy Lungs, School of Biomedical Sciences and Pharmacy, Faculty of Health, Hunter Medical Research Institute, University of Newcastle, Callaghan, NSW 2300, Australia; Medical Research Centre, First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan, 450052, China; Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, Henan, 450052, China
推荐引用方式
GB/T 7714
Wang L.,Netto K.G.,Zhou L.,et al. Single-cell transcriptomic analysis reveals the immune landscape of lung in steroid-resistant asthma exacerbation[J],2021,118(2).
APA Wang L..,Netto K.G..,Zhou L..,Liu X..,Wang M..,...&Yang M..(2021).Single-cell transcriptomic analysis reveals the immune landscape of lung in steroid-resistant asthma exacerbation.Proceedings of the National Academy of Sciences of the United States of America,118(2).
MLA Wang L.,et al."Single-cell transcriptomic analysis reveals the immune landscape of lung in steroid-resistant asthma exacerbation".Proceedings of the National Academy of Sciences of the United States of America 118.2(2021).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang L.]的文章
[Netto K.G.]的文章
[Zhou L.]的文章
百度学术
百度学术中相似的文章
[Wang L.]的文章
[Netto K.G.]的文章
[Zhou L.]的文章
必应学术
必应学术中相似的文章
[Wang L.]的文章
[Netto K.G.]的文章
[Zhou L.]的文章
相关权益政策
暂无数据
收藏/分享

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。