气候变化领域集成服务门户(CCPortal): Sparseness and smoothness regularized imaging for improving the resolution of Cryo-EM single-particle reconstruction

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DOI	10.1073/pnas.2013756118
	Sparseness and smoothness regularized imaging for improving the resolution of Cryo-EM single-particle reconstruction
	Luo Z.; Campos-Acevedo A.A.; Lv L.; Wang Q.; Ma J.
发表日期	2021
ISSN	00278424
卷号	118 期号:2
英文摘要	In this paper, we present a refinement method for cryo-electron microscopy (cryo-EM) single-particle reconstruction, termed as OPUS-SSRI (Sparseness and Smoothness Regularized Imaging). In OPUS-SSRI, spatially varying sparseness and smoothness priors are incorporated to improve the regularity of electron density map, and a type of real space penalty function is designed. Moreover, we define the back-projection step as a local kernel regression and propose a first-order method to solve the resulting optimization problem. On the seven cryo-EM datasets that we tested, the average improvement in resolution by OPUS-SSRI over that from RELION 3.0, the commonly used image-processing software for single-particle cryo-EM, was 0.64 Å, with the largest improvement being 1.25 Å. We expect OPUS-SSRI to be an invaluable tool to the broad field of cryo-EM single-particle analysis. The implementation of OPUS-SSRI can be found at https://github.com/alncat/cryoem. © This open access article is distributed under Creative Commons Attribution-NonCommercialNoDerivatives License 4.0 (CC BY-NC-ND).
英文关键词	3D reconstruction; Cryo-EM; Ill-posed inverse problem; Smoothness; Sparseness
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181032
作者单位	Department of Bioengineering, Rice University, Houston, TX 77030, United States; Verna and Marrs Mclean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, United States; Multiscale Research Institute of Complex Systems, Fudan University, Shanghai, 201203, China
推荐引用方式 GB/T 7714	Luo Z.,Campos-Acevedo A.A.,Lv L.,et al. Sparseness and smoothness regularized imaging for improving the resolution of Cryo-EM single-particle reconstruction[J],2021,118(2).
APA	Luo Z.,Campos-Acevedo A.A.,Lv L.,Wang Q.,&Ma J..(2021).Sparseness and smoothness regularized imaging for improving the resolution of Cryo-EM single-particle reconstruction.Proceedings of the National Academy of Sciences of the United States of America,118(2).
MLA	Luo Z.,et al."Sparseness and smoothness regularized imaging for improving the resolution of Cryo-EM single-particle reconstruction".Proceedings of the National Academy of Sciences of the United States of America 118.2(2021).