气候变化领域集成服务门户(CCPortal): An immunohistochemical study of lymphatic elements in the human brain

CCPortal

DOI	10.1073/PNAS.2002574118
	An immunohistochemical study of lymphatic elements in the human brain
	Mezey É.; Szalayova I.; Hogden C.T.; Brady A.; Dósa Á.; Sótonyi P.; Palkovits M.
发表日期	2021
ISSN	00278424
卷号	118 期号:3
英文摘要	Almost 150 papers about brain lymphatics have been published in the last 150 years. Recently, the information in these papers has been synthesized into a picture of central nervous system (CNS) “glymphatics,” but the fine structure of lymphatic elements in the human brain based on imaging specific markers of lymphatic endothelium has not been described. We used LYVE1 and PDPN antibodies to visualize lymphatic marker-positive cells (LMPCs) in postmortem human brain samples, meninges, cavernous sinus (cavum trigeminale), and cranial nerves and bolstered our findings with a VEGFR3 antibody. LMPCs were present in the perivascular space, the walls of small and large arteries and veins, the media of large vessels along smooth muscle cell membranes, and the vascular adventitia. Lymphatic marker staining was detected in the pia mater, in the arachnoid, in venous sinuses, and among the layers of the dura mater. There were many LMPCs in the perineurium and endoneurium of cranial nerves. Soluble waste may move from the brain parenchyma via perivascular and paravascular routes to the closest subarachnoid space and then travel along the dura mater and/or cranial nerves. Particulate waste products travel along the laminae of the dura mater toward the jugular fossa, lamina cribrosa, and perineurium of the cranial nerves to enter the cervical lymphatics. CD3-positive T cells appear to be in close proximity to LMPCs in perivascular/perineural spaces throughout the brain. Both immunostaining and qPCR confirmed the presence of adhesion molecules in the CNS known to be involved in T cell migration. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cranial nerves; CSF; Meninges; Podoplanin; T cells
语种	英语
scopus关键词	CD3 antigen; microRNA; Article; blood vessel wall; brain; cavernous sinus; controlled study; cranial nerve; dura mater; endoneurium; endothelium cell; human; human cell; human tissue; immunohistochemistry; incubation time; jugular fossa; lamina cribrosa; lymphatic endothelial cell; lymphocyte migration; meninx; perineurium; perivascular space; priority journal; real time polymerase chain reaction; staining; subarachnoid space; T lymphocyte
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/181010
作者单位	Adult Stem Cell Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, United States; Department of Forensic Sciences, Semmelweis University, Budapest, H-1091, Hungary; Human Brain Tissue Bank, Semmelweis University, Budapest, H-1094, Hungary
推荐引用方式 GB/T 7714	Mezey É.,Szalayova I.,Hogden C.T.,et al. An immunohistochemical study of lymphatic elements in the human brain[J],2021,118(3).
APA	Mezey É..,Szalayova I..,Hogden C.T..,Brady A..,Dósa Á..,...&Palkovits M..(2021).An immunohistochemical study of lymphatic elements in the human brain.Proceedings of the National Academy of Sciences of the United States of America,118(3).
MLA	Mezey É.,et al."An immunohistochemical study of lymphatic elements in the human brain".Proceedings of the National Academy of Sciences of the United States of America 118.3(2021).