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DOI10.1073/pnas.2005134118
The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion
Liu J.; Jang J.Y.; Pirooznia M.; Liu S.; Finkel T.
发表日期2021
ISSN00278424
卷号118期号:3
英文摘要At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should, therefore, be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers. © 2021 National Academy of Sciences. All rights reserved.
英文关键词Protein secretion | biotinylation | mouse model
语种英语
scopus关键词biotin; cell protein; cre recombinase; secretome; unclassified drug; animal experiment; animal tissue; Article; biotinylation; controlled study; endoplasmic reticulum; exercise; female; human; human cell; in vivo study; male; mass spectrometry; mouse; nonhuman; priority journal; protein analysis; protein blood level; protein expression; protein secretion; stress
来源期刊Proceedings of the National Academy of Sciences of the United States of America
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/181001
作者单位Aging Institute, University of Pittsburgh School of Medicine, UPMC, Pittsburgh, PA 15219, United States; Department of Medicine, Division of Cardiology, University ofPittsburgh/UPMC, Pittsburgh, PA 15219, United States; Bioinformatics and Computational Core Facility, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, United States; Department of Medicine, Division of Infectious Disease, University of Pittsburgh/UPMC, Pittsburgh, PA 15219, United States
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Liu J.,Jang J.Y.,Pirooznia M.,et al. The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion[J],2021,118(3).
APA Liu J.,Jang J.Y.,Pirooznia M.,Liu S.,&Finkel T..(2021).The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.Proceedings of the National Academy of Sciences of the United States of America,118(3).
MLA Liu J.,et al."The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion".Proceedings of the National Academy of Sciences of the United States of America 118.3(2021).
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