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DOI | 10.1073/pnas.2005134118 |
The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion | |
Liu J.; Jang J.Y.; Pirooznia M.; Liu S.; Finkel T. | |
发表日期 | 2021 |
ISSN | 00278424 |
卷号 | 118期号:3 |
英文摘要 | At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should, therefore, be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers. © 2021 National Academy of Sciences. All rights reserved. |
英文关键词 | Protein secretion | biotinylation | mouse model |
语种 | 英语 |
scopus关键词 | biotin; cell protein; cre recombinase; secretome; unclassified drug; animal experiment; animal tissue; Article; biotinylation; controlled study; endoplasmic reticulum; exercise; female; human; human cell; in vivo study; male; mass spectrometry; mouse; nonhuman; priority journal; protein analysis; protein blood level; protein expression; protein secretion; stress |
来源期刊 | Proceedings of the National Academy of Sciences of the United States of America |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/181001 |
作者单位 | Aging Institute, University of Pittsburgh School of Medicine, UPMC, Pittsburgh, PA 15219, United States; Department of Medicine, Division of Cardiology, University ofPittsburgh/UPMC, Pittsburgh, PA 15219, United States; Bioinformatics and Computational Core Facility, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, United States; Department of Medicine, Division of Infectious Disease, University of Pittsburgh/UPMC, Pittsburgh, PA 15219, United States |
推荐引用方式 GB/T 7714 | Liu J.,Jang J.Y.,Pirooznia M.,et al. The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion[J],2021,118(3). |
APA | Liu J.,Jang J.Y.,Pirooznia M.,Liu S.,&Finkel T..(2021).The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion.Proceedings of the National Academy of Sciences of the United States of America,118(3). |
MLA | Liu J.,et al."The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion".Proceedings of the National Academy of Sciences of the United States of America 118.3(2021). |
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