气候变化领域集成服务门户(CCPortal): Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity

CCPortal

DOI	10.1073/pnas.2004670118
	Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity
	Rayapuram N.; Jarad M.; Alhoraibi H.M.; Bigeard J.; Abulfaraj A.A.; Völz R.; Mariappan K.G.; Almeida-Trapp M.; Schlöffel M.; Lastrucci E.; Bonhomme L.; Gust A.A.; Mithöfer A.; Arold S.T.; Pflieger D.; Hirt H.
发表日期	2021
ISSN	00278424
卷号	118 期号:3
英文摘要	In many eukaryotic systems during immune responses, mitogen-activated protein kinases (MAPKs) link cytoplasmic signaling to chromatin events by targeting transcription factors, chromatin remodeling complexes, and the RNA polymerase machinery. So far, knowledge on these events is scarce in plants and no attempts have been made to focus on phosphorylation events of chromatin-associated proteins. Here we carried out chromatin phosphoproteomics upon elicitor-induced activation of Arabidopsis. The events in WT were compared with those in mpk3, mpk4, and mpk6 mutant plants to decipher specific MAPK targets. Our study highlights distinct signaling networks involving MPK3, MPK4, and MPK6 in chromatin organization and modification, as well as in RNA transcription and processing. Among the chromatin targets, we characterized the AT-hook motif containing nuclear localized (AHL) DNA-binding protein AHL13 as a substrate of immune MAPKs. AHL13 knockout mutant plants are compromised in pathogen-associated molecular pattern (PAMP)-induced reactive oxygen species production, expression of defense genes, and PAMP-triggered immunity. Transcriptome analysis revealed that AHL13 regulates key factors of jasmonic acid biosynthesis and signaling and affects immunity toward Pseudomonas syringae and Botrytis cinerea pathogens. Mutational analysis of the phosphorylation sites of AHL13 demonstrated that phosphorylation regulates AHL13 protein stability and thereby its immune functions. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Phosphoproteomics \| MAPK signaling \| chromatin \| immunity \| AT-hook motif transcription factor
语种	英语
scopus关键词	DNA binding protein; jasmonic acid; mitogen activated protein kinase; pathogen associated molecular pattern; protein AHL13; reactive oxygen metabolite; unclassified drug; Arabidopsis; Article; AT hook motif; Botrytis cinerea; chromatin; comparative study; controlled study; gene expression; immune response; mpk3 gene; mpk4 gene; mpk6 gene; mutational analysis; nonhuman; nuclear localization signal; phosphoproteomics; plant gene; priority journal; protein phosphorylation; protein stability; Pseudomonas syringae; RNA processing; RNA transcription; signal transduction; transcriptomics
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180946
作者单位	Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, 23955, Saudi Arabia; Centre for Research in Agricultural Genomics, Consejo Superior de Investigaciones Cientificas, Catalan Institute for Food and Agricultural Research and Technology, Autonomous University of Barcelona, University of Barcelona, Barcelona, 08007, Spain; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, 21551, Saudi Arabia; CNRS, Institut National de Recherche pour l’Agriculture, l’Alimentation, et l’Environnement, University Evry, Institute of Plant Sciences Paris-Saclay, Université Paris-Saclay, Orsay, 91405, France; Institut National de Recherche pour l’Agriculture, l’Alimentation, et l’Environnement, Institute of Plant Sciences Paris-Saclay, Université de Paris, CNRS, Orsay, 91405, France; Department of Biological Sciences, College of Sciences and Arts-Rabigh Campus, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; Plant ...
推荐引用方式 GB/T 7714	Rayapuram N.,Jarad M.,Alhoraibi H.M.,et al. Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity[J],2021,118(3).
APA	Rayapuram N..,Jarad M..,Alhoraibi H.M..,Bigeard J..,Abulfaraj A.A..,...&Hirt H..(2021).Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity.Proceedings of the National Academy of Sciences of the United States of America,118(3).
MLA	Rayapuram N.,et al."Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity".Proceedings of the National Academy of Sciences of the United States of America 118.3(2021).