气候变化领域集成服务门户(CCPortal): Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex

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DOI	10.1073/pnas.2019163118
	Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex
	Xie Y.; Lord C.L.; Clarke B.P.; Ivey A.L.; Hill P.S.; Hayes McDonald W.; Wente S.R.; Ren Y.
发表日期	2021
ISSN	00278424
卷号	118 期号:3
英文摘要	The C-terminal domain (CTD) kinase I (CTDK-1) complex is the primary RNA Polymerase II (Pol II) CTD Ser2 kinase in budding yeast. CTDK-1 consists of a cyclin-dependent kinase (CDK) Ctk1, a cyclin Ctk2, and a unique subunit Ctk3 required for CTDK-1 activity. Here, we present a crystal structure of CTDK-1 at 1.85-Å resolution. The structure reveals that, compared to the canonical two-component CDK-cyclin system, the third component Ctk3 of CTDK-1 plays a critical role in Ctk1 activation by stabilizing a key element of CDK regulation, the T-loop, in an active conformation. In addition, Ctk3 contributes to the assembly of CTDK-1 through extensive interactions with both Ctk1 and Ctk2. We also demonstrate that CTDK-1 physically and genetically interacts with the serine/arginine-like protein Gbp2. Together, the data in our work reveal a regulatory mechanism of CDK complexes. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cyclin-dependent kinase \| X-ray crystallography \| transcription \| mRNA processing
语种	英语
scopus关键词	cyclin dependent kinase; cycline; fungal RNA; protein C terminal domain kinase 1; protein Ctk1; protein Ctk3; protein Gbp2; RNA polymerase II; serine arginine rich protein; unclassified drug; Article; crystal structure; enzyme activation; enzyme mechanism; enzyme regulation; nonhuman; priority journal; protein conformation; protein protein interaction; protein stability; protein structure
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180945
作者单位	Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, United States; Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, United States; Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, TN 37232, United States
推荐引用方式 GB/T 7714	Xie Y.,Lord C.L.,Clarke B.P.,et al. Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex[J],2021,118(3).
APA	Xie Y..,Lord C.L..,Clarke B.P..,Ivey A.L..,Hill P.S..,...&Ren Y..(2021).Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex.Proceedings of the National Academy of Sciences of the United States of America,118(3).
MLA	Xie Y.,et al."Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex".Proceedings of the National Academy of Sciences of the United States of America 118.3(2021).