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DOI	10.1073/pnas.2014592118
	Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation
	Kozlowski R.; Zhao J.; Brian Dyer R.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	We have studied the role of protein dynamics in chemical catalysis in the enzyme dihydrofolate reductase (DHFR), using a pump–probe method that employs pulsed-laser photothermal heating of a gold nanoparticle (AuNP) to directly excite a local region of the protein structure and transient absorbance to probe the effect on enzyme activity. Enzyme activity is accelerated by pulsed-laser excitation when the AuNP is attached close to a network of coupled motions in DHFR (on the FG loop, containing residues 116–132, or on a nearby alpha helix). No rate acceleration is observed when the AuNP is attached away from the network (distal mutant and His-tagged mutant) with pulsed excitation, or for any attachment site with continuous wave excitation. We interpret these results within an energy landscape model in which transient, site-specific addition of energy to the enzyme speeds up the search for reactive conformations by activating motions that facilitate this search. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Dynamics; Enzyme; Nanoparticles; Photothermal
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180917
作者单位	Department of Chemistry, Emory University, Atlanta, GA 30322, United States
推荐引用方式 GB/T 7714	Kozlowski R.,Zhao J.,Brian Dyer R.. Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation[J],2021,118(4).
APA	Kozlowski R.,Zhao J.,&Brian Dyer R..(2021).Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Kozlowski R.,et al."Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).