气候变化领域集成服务门户(CCPortal): Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II

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DOI	10.1073/pnas.2019246118
	Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II
	Kumar A.; Prasad A.; Sedlárová M.; Kale R.; Frankel L.K.; Sallans L.; Bricker T.M.; Pospíšil P.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	Photosystem II (PSII) is an intrinsic membrane protein complex that functions as a light-driven water:plastoquinone oxidoreductase in oxygenic photosynthesis. Electron transport in PSII is associated with formation of reactive oxygen species (ROS) responsible for oxidative modifications of PSII proteins. In this study, oxidative modifications of the D1 and D2 proteins by the superoxide anion (O2•−) and the hydroxyl (HO•) radicals were studied in WT and a tocopherol cyclase (vte1) mutant, which is deficient in the lipid-soluble antioxidant α-tocopherol. In the absence of this antioxidant, high-resolution tandem mass spectrometry was used to identify oxidation of D1:130E to hydroxyglutamic acid by O2•− at the PheoD1 site. Additionally, D1:246Y was modified to either tyrosine hydroperoxide or dihydroxyphenylalanine by O2•− and HO•, respectively, in the vicinity of the nonheme iron. We propose that α-tocopherol is localized near PheoD1 and the nonheme iron, with its chromanol head exposed to the lipid–water interface. This helps to prevent oxidative modification of the amino acid’s hydrogen that is bonded to PheoD1 and the nonheme iron (via bicarbonate), and thus protects electron transport in PSII from ROS damage. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	EPR; Mass spectrometry; Photosystem II; Reactive oxygen species; Tocopherol
语种	英语
scopus关键词	alpha tocopherol; amino acid; bicarbonate; carbonyl derivative; DOPA; hydroperoxide; hydroxyglutamic acid; hydroxyl group; iron; lyase; oxygen radical; reactive oxygen metabolite; superoxide; tocopherol; tocopherol cyclase; tyrosine hydroperoxide; unclassified drug; amino acid metabolism; amino acid oxidation; Arabidopsis; Article; controlled study; electron transport; hydrogen bond; nonhuman; oxidation; photosystem II; plant leaf; priority journal; tandem mass spectrometry; thylakoid membrane
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180896
作者单位	Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 771 47, Czech Republic; Department of Botany, Faculty of Science, Palacký University, Olomouc, 771 47, Czech Republic; Department of Biological Sciences, Division of Biochemistry and Molecular Biology, Louisiana State University, Baton Rouge, LA 70803, United States; Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, United States
推荐引用方式 GB/T 7714	Kumar A.,Prasad A.,Sedlárová M.,et al. Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II[J],2021,118(4).
APA	Kumar A..,Prasad A..,Sedlárová M..,Kale R..,Frankel L.K..,...&Pospíšil P..(2021).Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Kumar A.,et al."Tocopherol controls D1 amino acid oxidation by oxygen radicals in Photosystem II".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).