气候变化领域集成服务门户(CCPortal): Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels

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DOI	10.1073/pnas.2014194118
	Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels
	Batalov I.; Stevens K.R.; DeForest C.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	Hydrogel biomaterials derived from natural biopolymers (e.g., fibrin, collagen, decellularized extracellular matrix) are regularly utilized in three-dimensional (3D) cell culture and tissue engineering. In contrast to those based on synthetic polymers, natural materials permit enhanced cytocompatibility, matrix remodeling, and biological integration. Despite these advantages, natural protein-based gels have lagged behind synthetic alternatives in their tunability; methods to selectively modulate the biochemical properties of these networks in a user-defined and heterogeneous fashion that can drive encapsulated cell function have not yet been established. Here, we report a generalizable strategy utilizing a photomediated oxime ligation to covalently decorate naturally derived hydrogels with bioactive proteins including growth factors. This bioorthogonal photofunctionalization is readily amenable to mask-based and laser-scanning lithographic patterning, enabling full four-dimensional (4D) control over protein immobilization within virtually any natural protein-based biomaterial. Such versatility affords exciting opportunities to probe and direct advanced cell fates inaccessible using purely synthetic approaches in response to anisotropic environmental signaling. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	4D biology; Biomaterials; Hydrogel; Photochemistry; Protein
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180893
作者单位	Department of Chemical Engineering, University of Washington, Seattle, WA 98105, United States; Department of Bioengineering, University of Washington, Seattle, WA 98105, United States; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, United States; Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, WA 98195, United States; Molecular Engineering & Sciences Institute, University of Washington, Seattle, WA 98105, United States; Department of Chemistry, University of Washington, Seattle, WA 98105, United States
推荐引用方式 GB/T 7714	Batalov I.,Stevens K.R.,DeForest C.A.. Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels[J],2021,118(4).
APA	Batalov I.,Stevens K.R.,&DeForest C.A..(2021).Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Batalov I.,et al."Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).