气候变化领域集成服务门户(CCPortal): A short ORF-encoded transcriptional regulator

CCPortal

DOI	10.1073/pnas.2021943118
	A short ORF-encoded transcriptional regulator
	Koh M.; Ahmad I.; Ko Y.; Zhang Y.; Martinez T.F.; Diedrich J.K.; Chu Q.; Moresco J.J.; Erb M.A.; Saghatelian A.; Schultz P.G.; Bollong M.J.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	Recent technological advances have expanded the annotated protein coding content of mammalian genomes, as hundreds of previously unidentified, short open reading frame (ORF)-encoded peptides (SEPs) have now been found to be translated. Although several studies have identified important physiological roles for this emerging protein class, a general method to define their interactomes is lacking. Here, we demonstrate that genetic incorporation of the photo-crosslinking noncanonical amino acid AbK into SEP transgenes allows for the facile identification of SEP cellular interaction partners using affinity-based methods. From a survey of seven SEPs, we report the discovery of short ORF-encoded histone binding protein (SEHBP), a conserved microprotein that interacts with chromatin-associated proteins, localizes to discrete genomic loci, and induces a robust transcriptional program when overexpressed in human cells. This work affords a straightforward method to help define the physiological roles of SEPs and demonstrates its utility by identifying SEHBP as a short ORF-encoded transcription factor. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Expanded genetic code; Photo-crosslinking; Short open reading frame-encoded peptide; Transcriptional regulation
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180876
作者单位	Department of Chemistry, Scripps Research Institute, San diego, CA 92037, United States; Department of Chemistry, Pusan National University, Busan, 46241, South Korea; Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, San diego, CA 92037, United States
推荐引用方式 GB/T 7714	Koh M.,Ahmad I.,Ko Y.,et al. A short ORF-encoded transcriptional regulator[J],2021,118(4).
APA	Koh M..,Ahmad I..,Ko Y..,Zhang Y..,Martinez T.F..,...&Bollong M.J..(2021).A short ORF-encoded transcriptional regulator.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Koh M.,et al."A short ORF-encoded transcriptional regulator".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).