气候变化领域集成服务门户(CCPortal): Longitudinal shear stress response in human endothelial cells to atheroprone and atheroprotective conditions

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DOI	10.1073/pnas.2023236118
	Longitudinal shear stress response in human endothelial cells to atheroprone and atheroprotective conditions
	Maurya M.R.; Gupta S.; Li J.Y.-S.; Ajami N.E.; Chen Z.B.; Shyy J.Y.-J.; Chien S.; Subramaniam S.
发表日期	2021
ISSN	00278424
卷号	118 期号:4
英文摘要	The two main blood flow patterns, namely, pulsatile shear (PS) prevalent in straight segments of arteries and oscillatory shear (OS) observed at branch points, are associated with atheroprotective (healthy) and atheroprone (unhealthy) vascular phenotypes, respectively. The effects of blood flow-induced shear stress on endothelial cells (ECs) and vascular health have generally been studied using human umbilical vein endothelial cells (HUVECs). While there are a few studies comparing the differential roles of PS and OS across different types of ECs at a single time point, there is a paucity of studies comparing the temporal responses between different EC types. In the current study, we measured OS and PS transcriptomic responses in human aortic endothelial cells (HAECs) over 24 h and compared these temporal responses of HAECs with our previous findings on HUVECs. The measurements were made at 1, 4, and 24 h in order to capture the responses at early, mid, and late time points after shearing. The results indicate that the responses of HAECs and HUVECs are qualitatively similar for endothelial function-relevant genes and several important pathways with a few exceptions, thus demonstrating that HUVECs can be used as a model to investigate the effects of shear on arterial ECs, with consideration of the differences. Our findings show that HAECs exhibit an earlier response or faster kinetics as compared to HUVECs. The comparative analysis of HAECs and HUVECs presented here offers insights into the mechanisms of common and disparate shear stress responses across these two major endothelial cell types. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Atherosclerosis; Endothelial cells; Systems biology; Temporal analysis of flow response; Transcriptomics
语种	英语
scopus关键词	aortic endothelial cell; Article; blood flow; cell function; cell protection; endothelium cell; genetic association; human; human cell; kinetics; oscillatory shear; priority journal; pulsatile shear; qualitative analysis; shear stress; signal transduction; temporal analysis; time factor; transcriptomics; umbilical vein endothelial cell
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180858
作者单位	Department of Bioengineering, University of California San Diego, San diego, CA 92093, United States; San Diego Supercomputer Center, University of California San Diego, San diego, CA 92093, United States; Institute of Engineering in Medicine, University of California San Diego, San diego, CA 92093, United States; Bioinformatics and Systems Biology Graduate Program, University of California San Diego, San diego, CA 92023, United States; Department of Diabetes Complications and Metabolism, Beckman Research Institute, City of Hope, CA 91010, United States; Department of Medicine, University of California San Diego, San diego, CA 92093, United States; Department of Computer Science and Engineering, University of California San Diego, San diego, CA 92093, United States; Department of Cellular and Molecular Medicine, University of California San Diego, San diego, CA 92093, United States
推荐引用方式 GB/T 7714	Maurya M.R.,Gupta S.,Li J.Y.-S.,et al. Longitudinal shear stress response in human endothelial cells to atheroprone and atheroprotective conditions[J],2021,118(4).
APA	Maurya M.R..,Gupta S..,Li J.Y.-S..,Ajami N.E..,Chen Z.B..,...&Subramaniam S..(2021).Longitudinal shear stress response in human endothelial cells to atheroprone and atheroprotective conditions.Proceedings of the National Academy of Sciences of the United States of America,118(4).
MLA	Maurya M.R.,et al."Longitudinal shear stress response in human endothelial cells to atheroprone and atheroprotective conditions".Proceedings of the National Academy of Sciences of the United States of America 118.4(2021).