气候变化领域集成服务门户(CCPortal): Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with Escherichia coli

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DOI	10.1073/pnas.2016886118
	Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with Escherichia coli
	Card K.J.; Thomas M.D.; Graves J.L.; Jr.; Barrick J.E.; Lenski R.E.
发表日期	2021
ISSN	00278424
卷号	118 期号:5
英文摘要	Antibiotic resistance is a growing health concern. Efforts to control resistance would benefit from an improved ability to forecast when and how it will evolve. Epistatic interactions between mutations can promote divergent evolutionary trajectories, which complicates our ability to predict evolution. We recently showed that differences between genetic backgrounds can lead to idiosyncratic responses in the evolvability of phenotypic resistance, even among closely related Escherichia coli strains. In this study, we examined whether a strain’s genetic background also influences the genotypic evolution of resistance. Do lineages founded by different genotypes take parallel or divergent mutational paths to achieve their evolved resistance states? We addressed this question by sequencing the complete genomes of antibiotic-resistant clones that evolved from several different genetic starting points during our earlier experiments. We first validated our statistical approach by quantifying the specificity of genomic evolution with respect to antibiotic treatment. As expected, mutations in particular genes were strongly associated with each drug. Then, we determined that replicate lines evolved from the same founding genotypes had more parallel mutations at the gene level than lines evolved from different founding genotypes, although these effects were more subtle than those showing antibiotic specificity. Taken together with our previous work, we conclude that historical contingency can alter both genotypic and phenotypic pathways to antibiotic resistance. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Antimicrobial resistance; Epistasis; Experimental evolution; Historical contingency; Parallel evolution
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180841
作者单位	BEACON Center for the Study of Evolution in Action, Michigan State University, East Lansing, MI 48824, United States; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States; Program in Ecology, Evolutionary Biology, and Behavior, Michigan State University, East Lansing, MI 48824, United States; Department of Biology, North Carolina A&T State University, Greensboro, NC 27411, United States; Department of Nanoengineering, Joint School of Nanoscience & Nanoengineering, North Carolina A&T State University, University of North Carolina Greensboro, Greensboro, NC 27401, United States; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, United States
推荐引用方式 GB/T 7714	Card K.J.,Thomas M.D.,Graves J.L.,et al. Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with Escherichia coli[J],2021,118(5).
APA	Card K.J.,Thomas M.D.,Graves J.L.,Jr.,Barrick J.E.,&Lenski R.E..(2021).Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America,118(5).
MLA	Card K.J.,et al."Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with Escherichia coli".Proceedings of the National Academy of Sciences of the United States of America 118.5(2021).