气候变化领域集成服务门户(CCPortal): Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus

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DOI	10.1073/pnas.2018053118
	Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus
	Huang G.; Xiao Y.; Pi X.; Zhao L.; Zhu Q.; Wang W.; Kuang T.; Han G.; Sui S.-F.; Shen J.-R.
发表日期	2021
ISSN	00278424
卷号	118 期号:5
英文摘要	Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyzes light-driven water oxidation, leading to the conversion of light energy into chemical energy and the release of molecular oxygen. Psb27 is a small thylakoid lumen-localized protein known to serve as an assembly factor for the biogenesis and repair of the PSII complex. The exact location and binding fashion of Psb27 in the intermediate PSII remain elusive. Here, we report the structure of a dimeric Psb27-PSII complex purified from a psbV deletion mutant (ΔPsbV) of the cyanobacterium Thermosynechococcus vulcanus, solved by cryo-electron microscopy. Our structure showed that Psb27 is associated with CP43 at the luminal side, with specific interactions formed between Helix 2 and Helix 3 of Psb27 and a loop region between Helix 3 and Helix 4 of CP43 (loop C) as well as the large, lumen-exposed and hydrophilic E-loop of CP43. The binding of Psb27 imposes some conflicts with the N-terminal region of PsbO and also induces some conformational changes in CP43, CP47, and D2. This makes PsbO unable to bind in the Psb27-PSII. Conformational changes also occurred in D1, PsbE, PsbF, and PsbZ; this, together with the conformational changes occurred in CP43, CP47, and D2, may prevent the binding of PsbU and induce dissociation of PsbJ. This structural information provides important insights into the regulation mechanism of Psb27 in the biogenesis and repair of PSII. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Assembly; Cryo-EM; Photosystem II; Psb27; Repair
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180814
作者单位	State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua University, Beijing, 100084, China; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong 518055, China; Research Institute for Interdisciplinary Science, Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan
推荐引用方式 GB/T 7714	Huang G.,Xiao Y.,Pi X.,et al. Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus[J],2021,118(5).
APA	Huang G..,Xiao Y..,Pi X..,Zhao L..,Zhu Q..,...&Shen J.-R..(2021).Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus.Proceedings of the National Academy of Sciences of the United States of America,118(5).
MLA	Huang G.,et al."Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus".Proceedings of the National Academy of Sciences of the United States of America 118.5(2021).