气候变化领域集成服务门户(CCPortal): GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior

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DOI	10.1073/pnas.2010168118
	GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior
	Maejima T.; Tsuno Y.; Miyazaki S.; Tsuneoka Y.; Hasegawa E.; Islam M.T.; Enoki R.; Nakamura T.J.; Mieda M.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	The suprachiasmatic nucleus (SCN), the central circadian pacemaker in mammals, is a network structure composed of multiple types of γ-aminobutyric acid (GABA)-ergic neurons and glial cells. However, the roles of GABA-mediated signaling in the SCN network remain controversial. Here, we report noticeable impairment of the circadian rhythm in mice with a specific deletion of the vesicular GABA transporter in arginine vasopressin (AVP)-producing neurons. These mice showed disturbed diurnal rhythms of GABAA receptor-mediated synaptic transmission in SCN neurons and marked lengthening of the activity time in circadian behavioral rhythms due to the extended interval between morning and evening locomotor activities. Synchrony of molecular circadian oscillations among SCN neurons did not significantly change, whereas the phase relationships between SCN molecular clocks and circadian morning/evening locomotor activities were altered significantly, as revealed by PER2::LUC imaging of SCN explants and in vivo recording of intracellular Ca2+ in SCN AVP neurons. In contrast, daily neuronal activity in SCN neurons in vivo clearly showed a bimodal pattern that correlated with dissociated morning/evening locomotor activities. Therefore, GABAergic transmission from AVP neurons regulates the timing of SCN neuronal firing to temporally restrict circadian behavior to appropriate time windows in SCN molecular clocks. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Arginine vasopressin; Circadian rhythm; GABA; Suprachiasmatic nucleus
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180766
作者单位	Department of Integrative Neurophysiology, Graduate School of Medical Sciences, Kanazawa University, Ishikawa, 920-8640, Japan; Laboratory of Animal Physiology, School of Agriculture, Meiji University, Kanagawa, 214-8571, Japan; Department of Anatomy, Faculty of Medicine, Toho University, Tokyo, 143-8540, Japan; Biophotonics Research Group, Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, 444-8787, Japan; Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, 444-8787, Japan; International Institute for Integrative Sleep Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan
推荐引用方式 GB/T 7714	Maejima T.,Tsuno Y.,Miyazaki S.,et al. GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior[J],2021,118(6).
APA	Maejima T..,Tsuno Y..,Miyazaki S..,Tsuneoka Y..,Hasegawa E..,...&Mieda M..(2021).GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Maejima T.,et al."GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).