气候变化领域集成服务门户(CCPortal): Neural and behavioral control in Caenorhabditis elegans by a yellow-light-activatable caged compound

CCPortal

DOI	10.1073/pnas.2009634118
	Neural and behavioral control in Caenorhabditis elegans by a yellow-light-activatable caged compound
	Takahashi H.; Kamiya M.; Kawatani M.; Umezawa K.; Ukita Y.; Niwa S.; Oda T.; Urano Y.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Caenorhabditis elegans is used as a model system to understand the neural basis of behavior, but application of caged compounds to manipulate and monitor the neural activity is hampered by the innate photophobic response of the nematode to short-wavelength light or by the low temporal resolution of photocontrol. Here, we develop boron dipyrromethene (BODIPY)-derived caged compounds that release bioactive phenol derivatives upon illumination in the yellow wavelength range. We show that activation of the transient receptor potential vanilloid 1 (TRPV1) cation channel by spatially targeted optical uncaging of the TRPV1 agonist N-vanillylnonanamide at 580 nm modulates neural activity. Further, neuronal activation by illumination-induced uncaging enables optical control of the behavior of freely moving C. elegans without inducing a photophobic response and without crosstalk between uncaging and simultaneous fluorescence monitoring of neural activity. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	C. elegans; Caged compounds; Fluorescence imaging; Photocontrol; Photolysis
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180761
作者单位	Graduate School of Medicine, University of Tokyo, Tokyo, 113-0033, Japan; Department of Anatomy and Structural Biology, Graduate School of Medicine, University of Yamanashi, Chuo, Yamanashi, 409-3898, Japan; Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), Kawaguchi, Saitama, 332-0012, Japan; Department of Mechanical Engineering, University of Yamanashi, Kofu, 400-8511, Japan; Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Aobaku, Sen-dai, Miyagi, 980-8578, Japan; Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113-0033, Japan; Core Research for Evolutional Science and Technology (CREST), Agency for Medical Research and Development (AMED), Chiyoda-ku, Tokyo, 100-0004, Japan
推荐引用方式 GB/T 7714	Takahashi H.,Kamiya M.,Kawatani M.,et al. Neural and behavioral control in Caenorhabditis elegans by a yellow-light-activatable caged compound[J],2021,118(6).
APA	Takahashi H..,Kamiya M..,Kawatani M..,Umezawa K..,Ukita Y..,...&Urano Y..(2021).Neural and behavioral control in Caenorhabditis elegans by a yellow-light-activatable caged compound.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Takahashi H.,et al."Neural and behavioral control in Caenorhabditis elegans by a yellow-light-activatable caged compound".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).