气候变化领域集成服务门户(CCPortal): Intercellular communication induces glycolytic synchronization waves between individually oscillating cells

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DOI	10.1073/pnas.2010075118
	Intercellular communication induces glycolytic synchronization waves between individually oscillating cells
	Mojica-Benavides M.; van Niekerk D.D.; Mijalkov M.; Snoep J.L.; Mehlig B.; Volpe G.; Goksör M.; Adiels C.B.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Many organs have internal structures with spatially differentiated and sometimes temporally synchronized groups of cells. The mechanisms leading to such differentiation and coordination are not well understood. Here we design a diffusion-limited microfluidic system to mimic a multicellular organ structure with peripheral blood flow and test whether a group of individually oscillating yeast cells could form subpopulations of spatially differentiated and temporally synchronized cells. Upon substrate addition, the dynamic response at single-cell level shows glycolytic oscillations, leading to wave fronts traveling through the monolayered population and to synchronized communities at well-defined positions in the cell chamber. A detailed mechanistic model with the architectural structure of the flow chamber incorporated successfully predicts the spatial-temporal experimental data, and allows for a molecular understanding of the observed phenomena. The intricate interplay of intracellular biochemical reaction networks leading to the oscillations, combined with intercellular communication via metabolic intermediates and fluid dynamics of the reaction chamber, is responsible for the generation of the subpopulations of synchronized cells. This mechanism, as analyzed from the model simulations, is experimentally tested using different concentrations of cyanide stress solutions. The results are reproducible and stable, despite cellular heterogeneity, and the spontaneous community development is reminiscent of a zoned cell differentiation often observed in multicellular organs. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell-cell communication; Glycolytic oscillations; Synchronization waves
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180744
作者单位	Department of Physics, University of Gothenburg, Gothenburg, SE-41296, Sweden; Department of Biochemistry, Stellenbosch University, Matieland, 7602, South Africa; Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm, SE-17177, Sweden; Molecular Cell Physiology, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, Netherlands
推荐引用方式 GB/T 7714	Mojica-Benavides M.,van Niekerk D.D.,Mijalkov M.,et al. Intercellular communication induces glycolytic synchronization waves between individually oscillating cells[J],2021,118(6).
APA	Mojica-Benavides M..,van Niekerk D.D..,Mijalkov M..,Snoep J.L..,Mehlig B..,...&Adiels C.B..(2021).Intercellular communication induces glycolytic synchronization waves between individually oscillating cells.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Mojica-Benavides M.,et al."Intercellular communication induces glycolytic synchronization waves between individually oscillating cells".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).