气候变化领域集成服务门户(CCPortal): Expression attenuation as a mechanism of robustness against gene duplication

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DOI	10.1073/pnas.2014345118
	Expression attenuation as a mechanism of robustness against gene duplication
	Ascencio D.; Diss G.; Gagnon-Arsenault I.; Dubé A.K.; DeLuna A.; Landry C.R.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Gene duplication is ubiquitous and a major driver of phenotypic diversity across the tree of life, but its immediate consequences are not fully understood. Deleterious effects would decrease the probability of retention of duplicates and prevent their contribution to long-term evolution. One possible detrimental effect of duplication is the perturbation of the stoichiometry of protein complexes. Here, we measured the fitness effects of the duplication of 899 essential genes in the budding yeast using high-resolution competition assays. At least 10% of genes caused a fitness disadvantage when duplicated. Intriguingly, the duplication of most protein complex subunits had small to nondetectable effects on fitness, with few exceptions. We selected four complexes with subunits that had an impact on fitness when duplicated and measured the impact of individual gene duplications on their protein-protein interactions. We found that very few duplications affect both fitness and interactions. Furthermore, large complexes such as the 26S proteasome are protected from gene duplication by attenuation of protein abundance. Regulatory mechanisms that maintain the stoichiometric balance of protein complexes may protect from the immediate effects of gene duplication. Our results show that a better understanding of protein regulation and assembly in complexes is required for the refinement of current models of gene duplication. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Dosage balance hypothesis; Fitness effects; Gene duplication; Gene expression; Protein interaction
语种	英语
scopus关键词	multiprotein complex; proteasome; Article; attenuation; budding yeast; competitive binding assay; controlled study; essential gene; gene dosage; gene duplication; gene expression; gene expression level; nonhuman; priority journal; protein assembly; protein protein interaction; regulatory mechanism; reproductive fitness; stoichiometry
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180714
作者单位	Regroupement Québécois de Recherche sur la Fonction, L'Ingénierie et les Applications des Protéines, Québec, QC G1V 0A6, Canada; Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC G1V 0A6, Canada; Centre de Recherche en Données Massives, L'Université Laval, Université Laval, Québec, QC G1V 0A6, Canada; Département de Biochimie, de Microbiologie et de Bio-informatique, Université Laval, Québec, QC G1V 0A6, Canada; Département de Biologie, Université Laval, Québec, QC G1V 0A6, Canada; Unidad de Genómica Avanzada (Langebio), Centro de Investigación y de Estudios Avanzados, Irapuato, Guanajuato, 36824, Mexico; Friedrich Miescher Institute for Biomedical Research, Basel, 4058, Switzerland
推荐引用方式 GB/T 7714	Ascencio D.,Diss G.,Gagnon-Arsenault I.,et al. Expression attenuation as a mechanism of robustness against gene duplication[J],2021,118(6).
APA	Ascencio D.,Diss G.,Gagnon-Arsenault I.,Dubé A.K.,DeLuna A.,&Landry C.R..(2021).Expression attenuation as a mechanism of robustness against gene duplication.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Ascencio D.,et al."Expression attenuation as a mechanism of robustness against gene duplication".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).