气候变化领域集成服务门户(CCPortal): Retinal bioavailability and functional effects of a synthetic very-long-chain polyunsaturated fatty acid in mice

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DOI	10.1073/pnas.2017739118
	Retinal bioavailability and functional effects of a synthetic very-long-chain polyunsaturated fatty acid in mice
	Gorusupudi A.; Rallabandi R.; Li B.; Arunkumar R.; David Blount J.; Rognon G.T.; Chang F.-Y.; Wade A.; Lucas S.; Conboy J.C.; Rainier J.D.; Bernstein P.S.
发表日期	2021
ISSN	00278424
卷号	118 期号:6
英文摘要	Rare, nondietary very-long-chain polyunsaturated fatty acids (VLC-PUFAs) are uniquely found in the retina and a few other vertebrate tissues. These special fatty acids play a clinically significant role in retinal degeneration and development, but their physiological and interventional research has been hampered because pure VLC-PUFAs are scarce. We hypothesize that if Stargardt-3 or age-related macular degeneration patients were to consume an adequate amount of VLC-PUFAs that could be directly used in the retina, it may be possible to bypass the steps of lipid elongation mediated by the retina's ELOVL4 enzyme and to delay or prevent degeneration. We report the synthesis of a VLC-PUFA (32:6 n-3) in sufficient quantity to study its bioavailability and functional benefits in the mouse retina. We acutely and chronically gavage fed wild-type mice and Elovl4 rod-cone conditional knockout mice this synthetic VLC-PUFA to understand its bioavailability and its role in visual function. VLC-PUFA-fed wild-type and Elovl4 conditional knockout mice show a significant increase in retinal VLC-PUFA levels in comparison to controls. The VLC-PUFA-fed mice also had improvement in the animals' visual acuity and electroretinography measurements. Further studies with synthetic VLC-PUFAs will continue to expand our understanding of the physiological roles of these unique retinal lipids, particularly with respect to their potential utility for the treatment and prevention of retinal degenerative diseases. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bioavailability; ELOVL4; Lipid; Retina; VLC-PUFA
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180677
作者单位	Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, Salt Lake City, UT 84132, United States; Department of Chemistry, University of Utah, Salt Lake City, UT 84112, United States
推荐引用方式 GB/T 7714	Gorusupudi A.,Rallabandi R.,Li B.,et al. Retinal bioavailability and functional effects of a synthetic very-long-chain polyunsaturated fatty acid in mice[J],2021,118(6).
APA	Gorusupudi A..,Rallabandi R..,Li B..,Arunkumar R..,David Blount J..,...&Bernstein P.S..(2021).Retinal bioavailability and functional effects of a synthetic very-long-chain polyunsaturated fatty acid in mice.Proceedings of the National Academy of Sciences of the United States of America,118(6).
MLA	Gorusupudi A.,et al."Retinal bioavailability and functional effects of a synthetic very-long-chain polyunsaturated fatty acid in mice".Proceedings of the National Academy of Sciences of the United States of America 118.6(2021).