气候变化领域集成服务门户(CCPortal): Characterization of the strain-rate–dependent mechanical response of single cell

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DOI	10.1073/pnas.2019347118
	Characterization of the strain-rate–dependent mechanical response of single cell–cell junctions
	Esfahani A.M.; Rosenbohm J.; Safa B.T.; Lavrik N.V.; Minnick G.; Zhou Q.; Kong F.; Jin X.; Kim E.; Liu Y.; Lu Y.; Lim J.Y.; Wahl J.K.; Dao M.; Huang C.; Yang R.
发表日期	2021
ISSN	00278424
卷号	118 期号:7
英文摘要	Cell–cell adhesions are often subjected to mechanical strains of different rates and magnitudes in normal tissue function. However, the rate-dependent mechanical behavior of individual cell–cell adhesions has not been fully characterized due to the lack of proper experimental techniques and therefore remains elusive. This is particularly true under large strain conditions, which may potentially lead to cell–cell adhesion dissociation and ultimately tissue fracture. In this study, we designed and fabricated a single-cell adhesion micro tensile tester (SCAμTT) using two-photon polymerization and performed displacement-controlled tensile tests of individual pairs of adherent epithelial cells with a mature cell–cell adhesion. Straining the cytoskeleton–cell adhesion complex system reveals a passive shear-thinning viscoelastic behavior and a rate-dependent active stress-relaxation mechanism mediated by cytoskeleton growth. Under low strain rates, stress relaxation mediated by the cytoskeleton can effectively relax junctional stress buildup and prevent adhesion bond rupture. Cadherin bond dissociation also exhibits rate-dependent strengthening, in which increased strain rate results in elevated stress levels at which cadherin bonds fail. This bond dissociation becomes a synchronized catastrophic event that leads to junction fracture at high strain rates. Even at high strain rates, a single cell–cell junction displays a remarkable tensile strength to sustain a strain as much as 200% before complete junction rupture. Collectively, the platform and the biophysical understandings in this study are expected to build a foundation for the mechanistic investigation of the adaptive viscoelasticity of the cell–cell junction. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell mechanics \| cell–cell junction \| stress–strain relationship \| stress relaxation
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180670
作者单位	Department of Mechanical and Materials Engineering, University of Nebraska–Lincoln, Lincoln, NE 68516, United States; Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6054, United States; School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore, 639798, Singapore; School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore; Department of Electrical and Computer Engineering, University of Nebraska–Lincoln, Lincoln, NE 68516, United States; Department of Oral Biology, University of Nebraska Medical Center, Lincoln, NE 68583, United States; Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637459, Singapore; Nebraska Center for Integrated Biomolecular Communication, University of Nebraska–Lincoln, Lincoln, NE 68588, United States...
推荐引用方式 GB/T 7714	Esfahani A.M.,Rosenbohm J.,Safa B.T.,等. Characterization of the strain-rate–dependent mechanical response of single cell–cell junctions[J],2021,118(7).
APA	Esfahani A.M..,Rosenbohm J..,Safa B.T..,Lavrik N.V..,Minnick G..,...&Yang R..(2021).Characterization of the strain-rate–dependent mechanical response of single cell–cell junctions.Proceedings of the National Academy of Sciences of the United States of America,118(7).
MLA	Esfahani A.M.,et al."Characterization of the strain-rate–dependent mechanical response of single cell–cell junctions".Proceedings of the National Academy of Sciences of the United States of America 118.7(2021).