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DOI	10.1073/pnas.2009475118
	The mop1 mutation affects the recombination landscape in maize
	Zhao M.; Ku J.-C.; Liu B.; Yang D.; Yin L.; Ferrell T.J.; Stoll C.E.; Guo W.; Zhang X.; Wang D.; Wang C.-J.R.; Lisch D.
发表日期	2021
ISSN	00278424
卷号	118 期号:7
英文摘要	Meiotic recombination is a fundamental process that generates genetic diversity and ensures the accurate segregation of homologous chromosomes. While a great deal is known about genetic factors that regulate recombination, relatively little is known about epigenetic factors, such as DNA methylation. In maize, we examined the effects on meiotic recombination of a mutation in a component of the RNA-directed DNA methylation pathway, Mop1 (Mediator of paramutation1), as well as a mutation in a component of the trans-acting small interference RNA biogenesis pathway, Lbl1 (Leafbladeless1). MOP1 is of particular interest with respect to recombination because it is responsible for methylation of transposable elements that are immediately adjacent to transcriptionally active genes. In the mop1 mutant, we found that meiotic recombination is uniformly decreased in pericentromeric regions but is generally increased in gene rich chromosomal arms.regions but is generally increased in gene rich chromosomal arms. This observation was further confirmed by cytogenetic analysis showing that although overall crossover numbers are unchanged, they occur more frequently in chromosomal arms in mop1 mutants. Using whole genome bisulfite sequencing, our data show that crossover redistribution is driven by loss of CHH (where H = A, T, or C) methylation within regions near genes. In contrast to what we observed in mop1 mutants, no significant changes were observed in the frequency of meiotic recombination in lbl1 mutants. Our data demonstrate that CHH methylation has a significant impact on the overall recombination landscape in maize despite its low frequency relative to CG and CHG methylation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Meiotic recombination \| DNA methylation \| mop1 \| lbl1
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180654
作者单位	Department of Biology, Miami University, Oxford, OH 45056, United States; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, 11529, Taiwan; Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, United States; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China; Division of Mathematics and Sciences, Delta State University, Cleveland, MS 38733, United States
推荐引用方式 GB/T 7714	Zhao M.,Ku J.-C.,Liu B.,et al. The mop1 mutation affects the recombination landscape in maize[J],2021,118(7).
APA	Zhao M..,Ku J.-C..,Liu B..,Yang D..,Yin L..,...&Lisch D..(2021).The mop1 mutation affects the recombination landscape in maize.Proceedings of the National Academy of Sciences of the United States of America,118(7).
MLA	Zhao M.,et al."The mop1 mutation affects the recombination landscape in maize".Proceedings of the National Academy of Sciences of the United States of America 118.7(2021).