气候变化领域集成服务门户(CCPortal): Chromatophores efficiently promote light-driven ATP synthesis and DNA transcription inside hybrid multicompartment artificial cells

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DOI	10.1073/pnas.2012170118
	Chromatophores efficiently promote light-driven ATP synthesis and DNA transcription inside hybrid multicompartment artificial cells
	Altamura E.; Albanese P.; Marotta R.; Milano F.; Fiore M.; Trotta M.; Stano P.; Mavelli F.
发表日期	2021
ISSN	00278424
卷号	118 期号:7
英文摘要	The construction of energetically autonomous artificial protocells is one of the most ambitious goals in bottom-up synthetic biology. Here, we show an efficient manner to build adenosine 5′-triphosphate (ATP) synthesizing hybrid multicompartment protocells. Bacterial chromatophores from Rhodobacter sphaeroides accomplish the photophosphorylation of adenosine 5′-diphosphate (ADP) to ATP, functioning as nanosized photosynthetic organellae when encapsulated inside artificial giant phospholipid vesicles (ATP production rate up to ∼100 ATP·s−1 per ATP synthase). The chromatophore morphology and the orientation of the photophosphorylation proteins were characterized by cryo-electron microscopy (cryo-EM) and time-resolved spectroscopy. The freshly synthesized ATP has been employed for sustaining the transcription of a DNA gene, following the RNA biosynthesis inside individual vesicles by confocal microscopy. The hybrid multicompartment approach here proposed is very promising for the construction of full-fledged artificial protocells because it relies on easy-to-obtain and ready-to-use chromatophores, paving the way for artificial simplified-autotroph protocells (ASAPs). © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Artificial protocells \| artificial photosynthesis \| bacterial chromatophores \| light transduction \| synthetic biology
语种	英语
scopus关键词	adenosine diphosphate; adenosine triphosphate; Article; artificial simplified autotroph protocell; bacterial chromatophore; biosynthesis; confocal microscopy; cryoelectron microscopy; DNA transcription; light driven ATP synthesis; nonhuman; phospholipid vesicle; photosynthetic phosphorylation; priority journal; protein structure; Rhodobacter sphaeroides; RNA synthesis; time resolved spectroscopy
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180624
作者单位	Chemistry Department, University of Bari Aldo Moro, Bari, 70125, Italy; Electron Microscopy Facility, Italian Institute of Technology, Genova, 16163, Italy; National Research Council, Institute of Sciences of Food Production, Ecotekne, Lecce, 73100, Italy; Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Université de Lyon “Claude Bernard Lyon 1, Villeurbanne Cedex, Lyon, 69622, France; CNR-IPCF Consiglio Nazionale delle Ricerche–Istituto per i Processi Chimico Fisici, Bari, 70125, Italy; Department of Biological and Environmental Sciences and Technologies, University of Salento, Ecotekne, Lecce, 73100, Italy; National Research Council–Institute of Nanotechnology, Bari, 70125, Italy
推荐引用方式 GB/T 7714	Altamura E.,Albanese P.,Marotta R.,et al. Chromatophores efficiently promote light-driven ATP synthesis and DNA transcription inside hybrid multicompartment artificial cells[J],2021,118(7).
APA	Altamura E..,Albanese P..,Marotta R..,Milano F..,Fiore M..,...&Mavelli F..(2021).Chromatophores efficiently promote light-driven ATP synthesis and DNA transcription inside hybrid multicompartment artificial cells.Proceedings of the National Academy of Sciences of the United States of America,118(7).
MLA	Altamura E.,et al."Chromatophores efficiently promote light-driven ATP synthesis and DNA transcription inside hybrid multicompartment artificial cells".Proceedings of the National Academy of Sciences of the United States of America 118.7(2021).