气候变化领域集成服务门户(CCPortal): Chemokine-biased robust self-organizing polarization of migrating cells in vivo

CCPortal

DOI	10.1073/pnas.2018480118
	Chemokine-biased robust self-organizing polarization of migrating cells in vivo
	Olguin-Olguin A.; Aalto A.; Maugis B.; Boquet-Pujadas A.; Hoffmann D.; Ermlich L.; Betz T.; Gov N.S.; Reichman-Fried M.; Raz E.
发表日期	2021
ISSN	00278424
卷号	118 期号:7
英文摘要	To study the mechanisms controlling front-rear polarity in migrating cells, we used zebrafish primordial germ cells (PGCs) as an in vivo model. We find that polarity of bleb-driven migrating cells can be initiated at the cell front, as manifested by actin accumulation at the future leading edge and myosin-dependent retrograde actin flow toward the other side of the cell. In such cases, the definition of the cell front, from which bleb-inhibiting proteins such as Ezrin are depleted, precedes the establishment of the cell rear, where those proteins accumulate. Conversely, following cell division, the accumulation of Ezrin at the cleavage plane is the first sign for cell polarity and this aspect of the cell becomes the cell back. Together, the antagonistic interactions between the cell front and back lead to a robust polarization of the cell. Furthermore, we show that chemokine signaling can bias the establishment of the front-rear axis of the cell, thereby guiding the migrating cells toward sites of higher levels of the attractant. We compare these results to a theoretical model according to which a critical value of actin treadmilling flow can initiate a positive feedback loop that leads to the generation of the front-rear axis and to stable cell polarization. Together, our in vivo findings and the mathematical model, provide an explanation for the observed nonoriented migration of primordial germ cells in the absence of the guidance cue, as well as for the directed migration toward the region where the gonad develops. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Amoeboid migration \| bleb \| cell polarity \| chemotaxis \| ezrin
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180623
作者单位	Institute of Cell Biology, Center for Molecular Biology of Inflammation, University of Münster, Münster, 48149, Germany; Bioimage Analysis Unit, Institut Pasteur, Paris, 75105, France; CNRS, UMR 3691, Paris, 75015, France; Sorbonne Université, Paris, 75005, France; Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, 76100, Israel
推荐引用方式 GB/T 7714	Olguin-Olguin A.,Aalto A.,Maugis B.,et al. Chemokine-biased robust self-organizing polarization of migrating cells in vivo[J],2021,118(7).
APA	Olguin-Olguin A..,Aalto A..,Maugis B..,Boquet-Pujadas A..,Hoffmann D..,...&Raz E..(2021).Chemokine-biased robust self-organizing polarization of migrating cells in vivo.Proceedings of the National Academy of Sciences of the United States of America,118(7).
MLA	Olguin-Olguin A.,et al."Chemokine-biased robust self-organizing polarization of migrating cells in vivo".Proceedings of the National Academy of Sciences of the United States of America 118.7(2021).