气候变化领域集成服务门户(CCPortal): Tissue folding at the organ–meristem boundary results in nuclear compression and chromatin compaction

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DOI	10.1073/pnas.2017859118
	Tissue folding at the organ–meristem boundary results in nuclear compression and chromatin compaction
	Fal K.; Korsbo N.; Alonso-Serra J.; Teles J.; Liu M.; Refahi Y.; Chabouté M.-E.; Jönsson H.; Hamant O.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Artificial mechanical perturbations affect chromatin in animal cells in culture. Whether this is also relevant to growing tissues in living organisms remains debated. In plants, aerial organ emergence occurs through localized outgrowth at the periphery of the shoot apical meristem, which also contains a stem cell niche. Interestingly, organ outgrowth has been proposed to generate compression in the saddle-shaped organ–meristem boundary domain. Yet whether such growth-induced mechanical stress affects chromatin in plant tissues is unknown. Here, by imaging the nuclear envelope in vivo over time and quantifying nucleus deformation, we demonstrate the presence of active nuclear compression in that domain. We developed a quantitative pipeline amenable to identifying a subset of very deformed nuclei deep in the boundary and in which nuclei become gradually narrower and more elongated as the cell contracts transversely. In this domain, we find that the number of chromocenters is reduced, as shown by chromatin staining and labeling, and that the expression of linker histone H1.3 is induced. As further evidence of the role of forces on chromatin changes, artificial compression with a MicroVice could induce the ectopic expression of H1.3 in the rest of the meristem. Furthermore, while the methylation status of chromatin was correlated with nucleus deformation at the meristem boundary, such correlation was lost in the h1.3 mutant. Altogether, we reveal that organogenesis in plants generates compression that is able to have global effects on chromatin in individual cells. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Chromatin; Mechanical forces; Nucleus; Organogenesis; Plant
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180530
作者单位	Laboratoire de Reproduction et Développement des Plantes, Université de Lyon, Université Claude Bernard Lyon 1, ENS de Lyon, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CNRS, Lyon Cedex 07, 69364, France; Department of Astronomy and Theoretical Physics, Lund University, Lund, SE22362, Sweden; Laboratoire Physiologie Cellulaire and Végétale, Institut de Biosciences et de Biotechnologie de Grenoble, Commissariat à l’Energie Atomique et aux Energies Alternatives–Grenoble, Grenoble cedex 9, 38054, France; Sainsbury Laboratory, University of Cambridge, Cambridge, CB2 1LR, United Kingdom; Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge, United Kingdom; Université de Reims Champagne Ardenne, INRAE, Fractionnement des Agro-Ressources et Environnement, UMR A 614, Reims, 51097, France; Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg Cedex, 67084, France
推荐引用方式 GB/T 7714	Fal K.,Korsbo N.,Alonso-Serra J.,等. Tissue folding at the organ–meristem boundary results in nuclear compression and chromatin compaction[J],2021,118(8).
APA	Fal K..,Korsbo N..,Alonso-Serra J..,Teles J..,Liu M..,...&Hamant O..(2021).Tissue folding at the organ–meristem boundary results in nuclear compression and chromatin compaction.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Fal K.,et al."Tissue folding at the organ–meristem boundary results in nuclear compression and chromatin compaction".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).