气候变化领域集成服务门户(CCPortal): Differential biosynthesis and cellular permeability explain longitudinal gibberellin gradients in growing roots

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DOI	10.1073/pnas.1921960118
	Differential biosynthesis and cellular permeability explain longitudinal gibberellin gradients in growing roots
	Rizza A.; Tang B.; Stanley C.E.; Grossmann G.; Owen M.R.; Band L.R.; Jones A.M.
发表日期	2021
ISSN	00278424
卷号	118 期号:8
英文摘要	Control over cell growth by mobile regulators underlies much of eukaryotic morphogenesis. In plant roots, cell division and elongation are separated into distinct longitudinal zones and both division and elongation are influenced by the growth regulatory hormone gibberellin (GA). Previously, a multicellular mathematical model predicted a GA maximum at the border of the meristematic and elongation zones. However, GA in roots was recently measured using a genetically encoded fluorescent biosensor, nlsGPS1, and found to be low in the meristematic zone grading to a maximum at the end of the elongation zone. Furthermore, the accumulation rate of exogenous GA was also found to be higher in the elongation zone. It was still unknown which biochemical activities were responsible for these mobile small molecule gradients and whether the spatiotemporal correlation between GA levels and cell length is important for root cell division and elongation patterns. Using a mathematical modeling approach in combination with high-resolution GA measurements in vivo, we now show how differentials in several biosynthetic enzyme steps contribute to the endogenous GA gradient and how differential cellular permeability contributes to an accumulation gradient of exogenous GA. We also analyzed the effects of altered GA distribution in roots and did not find significant phenotypes resulting from increased GA levels or signaling. We did find a substantial temporal delay between complementation of GA distribution and cell division and elongation phenotypes in a GA deficient mutant. Together, our results provide models of how GA gradients are directed and in turn direct root growth. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell growth; Gibberellin; Hormone biosensor; Mathematical modeling; Root development
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180519
作者单位	Sainsbury Laboratory, Cambridge University, Cambridge, CB2 1LR, United Kingdom; Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Zürich, 8093, Switzerland; Agroecology and Environment Research Division, Agroscope, Zürich, 8046, Switzerland; Centre for Organismal Studies, Heidelberg University, Heidelberg, 69120, Germany; Centre for Mathematical Medicine and Biology, School of Mathematical Sciences, University of Nottingham, Nottingham, NG7 2RD, United Kingdom; Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, United Kingdom
推荐引用方式 GB/T 7714	Rizza A.,Tang B.,Stanley C.E.,et al. Differential biosynthesis and cellular permeability explain longitudinal gibberellin gradients in growing roots[J],2021,118(8).
APA	Rizza A..,Tang B..,Stanley C.E..,Grossmann G..,Owen M.R..,...&Jones A.M..(2021).Differential biosynthesis and cellular permeability explain longitudinal gibberellin gradients in growing roots.Proceedings of the National Academy of Sciences of the United States of America,118(8).
MLA	Rizza A.,et al."Differential biosynthesis and cellular permeability explain longitudinal gibberellin gradients in growing roots".Proceedings of the National Academy of Sciences of the United States of America 118.8(2021).