气候变化领域集成服务门户(CCPortal): Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking

CCPortal

DOI	10.1073/pnas.2022133118
	Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking
	Park C.; Chen X.; Tian C.-L.; Park G.N.; Chenouard N.; Lee H.; Yeo X.Y.; Jung S.; Tsien R.W.; Bi G.-Q.; Park H.
发表日期	2021
ISSN	00278424
卷号	118 期号:9
英文摘要	Maintaining the balance between neuronal excitation and inhibition is essential for proper function of the central nervous system. Inhibitory synaptic transmission plays an important role in maintaining this balance. Although inhibitory transmission has higher kinetic demands compared to excitatory transmission, its properties are poorly understood. In particular, the dynamics and exocytosis of single inhibitory vesicles have not been investigated, due largely to both technical and practical limitations. Using a combination of quantum dots (QDs) conjugated to antibodies against the luminal domain of the vesicular GABA transporter to selectively label GABAergic (i.e., predominantly inhibitory) vesicles together with dual-focus imaging optics, we tracked the real-time three-dimensional position of single GABAergic vesicles up to the moment of exocytosis (i.e., fusion). Using three-dimensional trajectories, we found that GABAergic synaptic vesicles traveled a shorter distance prior to fusion and had a shorter time to fusion compared to synaptotagmin-1 (Syt1)-labeled vesicles, which were mostly from excitatory neurons. Moreover, our analysis revealed that GABAergic synaptic vesicles move more straightly to their release sites than Syt1-labeled vesicles. Finally, we found that GABAergic vesicles have a higher prevalence of kiss-and-run fusion than Syt1-labeled vesicles. These results indicate that inhibitory synaptic vesicles have a unique set of dynamics and exocytosis properties to support rapid synaptic inhibition, thereby maintaining a tightly regulated coordination between excitation and inhibition in the central nervous system. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Dynamics; Exocytosis; Inhibitory synaptic transmission; Synaptic vesicles; Three-dimensional tracking
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180461
作者单位	Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, 999077, Hong Kong; School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230052, China; Chinese Academy of Sciences, Key Laboratory of Brain Function and Disease, University of Science and Technology of China, Hefei, Anhui, 230052, China; Department of Chemistry, Chung-Ang University, Seoul, 06974, South Korea; Creative Research Initiative Center for Chemical Dynamics in Living Cells, Chung-Ang University, Seoul, 06974, South Korea; CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, University of Bordeaux, Bordeaux, F-33000, France; Institute of Medical Physics and Biophysics, University of Münster, Münster, 48149, Germany; Singapore Bioimaging Consortium, Agency for Science, Technology, and Research138667, Singapore; Department of Psychological Medicine, Yong Loo Lin School of Medicine, National University of Singapore117597, Singapore; Department of Phy...
推荐引用方式 GB/T 7714	Park C.,Chen X.,Tian C.-L.,et al. Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking[J],2021,118(9).
APA	Park C..,Chen X..,Tian C.-L..,Park G.N..,Chenouard N..,...&Park H..(2021).Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.Proceedings of the National Academy of Sciences of the United States of America,118(9).
MLA	Park C.,et al."Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking".Proceedings of the National Academy of Sciences of the United States of America 118.9(2021).