气候变化领域集成服务门户(CCPortal): Anomalous mechanics of Zn2+-modified fibrin networks

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DOI	10.1073/pnas.2020541118
	Anomalous mechanics of Zn2+-modified fibrin networks
	Xia J.; Cai L.-H.; Wu H.; MacKintosh F.C.; Weitz D.A.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Fibrin is the main component of blood clots. The mechanical properties of fibrin are therefore of critical importance in successful hemostasis. One of the divalent cations released by platelets during hemostasis is Zn2+; however, its effect on the network structure of fibrin gels and on the resultant mechanical properties remains poorly understood. Here, by combining mechanical measurements with three-dimensional confocal microscopy imaging, we show that Zn2+ can tune the fibrin network structure and alter its mechanical properties. In the presence of Zn2+, fibrin protofibrils form large bundles that cause a coarsening of the fibrin network due to an increase in fiber diameter and reduction of the total fiber length. We further show that the protofibrils in these bundles are loosely coupled to one another, which results in a decrease of the elastic modulus with increasing Zn2+ concentrations. We explore the elastic properties of these networks at both low and high stress: At low stress, the elasticity originates from pulling the thermal slack out of the network, and this is consistent with the thermal bending of the fibers. By contrast, at high stress, the elasticity exhibits a common master curve consistent with the stretching of individual protofibrils. These results show that the mechanics of a fibrin network are closely correlated with its microscopic structure and inform our understanding of the structure and physical mechanisms leading to defective or excessive clot stiffness. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Fibrin gel; Mechanics; Three-dimensional networks; Zn2+, bundle
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180400
作者单位	John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, United States; Department of Materials Science and Engineering, University of Virginia, Charlottesville, VA 22904, United States; Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX 77005, United States; Center for Theoretical Biological Physics, Rice University, Houston, TX 77030, United States; Department of Chemistry and Physics, Rice University, Houston, TX 77005, United States; Department of Astronomy, Rice University, Houston, TX 77005, United States; Department of Physics, Harvard University, Cambridge, MA 02138, United States
推荐引用方式 GB/T 7714	Xia J.,Cai L.-H.,Wu H.,et al. Anomalous mechanics of Zn2+-modified fibrin networks[J],2021,118(10).
APA	Xia J.,Cai L.-H.,Wu H.,MacKintosh F.C.,&Weitz D.A..(2021).Anomalous mechanics of Zn2+-modified fibrin networks.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Xia J.,et al."Anomalous mechanics of Zn2+-modified fibrin networks".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).