气候变化领域集成服务门户(CCPortal): SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants

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DOI	10.1073/pnas.2021351118
	SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants
	Verma V.; Srivastava A.K.; Gough C.; Campanaro A.; Srivastava M.; Morrell R.; Joyce J.; Bailey M.; Zhang C.; Krysan P.J.; Sadanandom A.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	The versatility of mitogen-activated protein kinases (MAPKs) in translating exogenous and endogenous stimuli into appropriate cellular responses depends on its substrate specificity. In animals, several mechanisms have been proposed about how MAPKs maintain specificity to regulate distinct functional pathways. However, little is known of mechanisms that enable substrate selectivity in plant MAPKs. Small ubiquitin-like modifier (SUMO), a posttranslational modification system, plays an important role in plant development and defense by rapid reprogramming of cellular events. In this study we identified a functional SUMO interaction motif (SIM) in Arabidopsis MPK3 and MPK6 that reveals a mechanism for selective interaction of MPK3/6 with SUMO-conjugated WRKY33, during defense. We show that WRKY33 is rapidly SUMOylated in response to Botrytis cinerea infection and flg22 elicitor treatment. SUMOylation mediates WRKY33 phosphorylation by MPKs and consequent transcription factor activity. Disruption of either WRKY33 SUMO or MPK3/6 SIM sites attenuates their interaction and inactivates WRKY33-mediated defense. However, MPK3/6 SIM mutants show normal interaction with a non-SUMOylated form of another transcription factor, SPEECHLESS, unraveling a role for SUMOylation in differential substrate selectivity by MPKs. We reveal that the SUMO proteases, SUMO PROTEASE RELATED TO FERTILITY1 (SPF1) and SPF2 control WRKY33 SUMOylation and demonstrate a role for these SUMO proteases in defense. Our data reveal a mechanism by which MPK3/6 prioritize molecular pathways by differentially selecting substrates using the SUMO–SIM module during defense responses. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Immunity; MAPKs; Plants; SUMO; WRKY33
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180387
作者单位	Department of Biosciences, Durham University, Durham, DH1 3LE, United Kingdom; Department of Horticulture, College of Agricultural and Life Science, University of Wisconsin–Madison, Madison, WI 53706, United States
推荐引用方式 GB/T 7714	Verma V.,Srivastava A.K.,Gough C.,et al. SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants[J],2021,118(10).
APA	Verma V..,Srivastava A.K..,Gough C..,Campanaro A..,Srivastava M..,...&Sadanandom A..(2021).SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Verma V.,et al."SUMO enables substrate selectivity by mitogen-activated protein kinases to regulate immunity in plants".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).