气候变化领域集成服务门户(CCPortal): Dynamics of hydraulic and contractile wave-mediated fluid transport during Drosophila oogenesis

CCPortal

DOI	10.1073/pnas.2019749118
	Dynamics of hydraulic and contractile wave-mediated fluid transport during Drosophila oogenesis
	Alsous J.I.; Romeo N.; Jackson J.A.; Mason F.M.; Dunkel J.; Martin A.C.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	From insects to mice, oocytes develop within cysts alongside nurse-like sister germ cells. Prior to fertilization, the nurse cells’ cytoplasmic contents are transported into the oocyte, which grows as its sister cells regress and die. Although critical for fertility, the biological and physical mechanisms underlying this transport process are poorly understood. Here, we combined live imaging of germline cysts, genetic perturbations, and mathematical modeling to investigate the dynamics and mechanisms that enable directional and complete cytoplasmic transport in Drosophila melanogaster egg chambers. We discovered that during “nurse cell (NC) dumping” most cytoplasm is transported into the oocyte independently of changes in myosin-II contractility, with dynamics instead explained by an effective Young–Laplace law, suggesting hydraulic transport induced by baseline cell-surface tension. A minimal flow-network model inspired by the famous two-balloon experiment and motivated by genetic analysis of a myosin mutant correctly predicts the directionality, intercellular pattern, and time scale of transport. Long thought to trigger transport through “squeezing,” changes in actomyosin contractility are required only once NC volume has become comparable to nuclear volume, in the form of surface contractile waves that drive NC dumping to completion. Our work thus demonstrates how biological and physical mechanisms cooperate to enable a critical developmental process that, until now, was thought to be mainly biochemically regulated. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Actomyosin; Hydraulic transport; Oogenesis
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180343
作者单位	Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Department of Physics, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Graduate Program in Biophysics, Harvard University, Cambridge, MA 02138, United States; Program in Cancer Biology, Vanderbilt University, Nashville, TN 37232, United States; Department of Mathematics, Massachusetts Institute of Technology, Cambridge, MA 02139, United States
推荐引用方式 GB/T 7714	Alsous J.I.,Romeo N.,Jackson J.A.,et al. Dynamics of hydraulic and contractile wave-mediated fluid transport during Drosophila oogenesis[J],2021,118(10).
APA	Alsous J.I.,Romeo N.,Jackson J.A.,Mason F.M.,Dunkel J.,&Martin A.C..(2021).Dynamics of hydraulic and contractile wave-mediated fluid transport during Drosophila oogenesis.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Alsous J.I.,et al."Dynamics of hydraulic and contractile wave-mediated fluid transport during Drosophila oogenesis".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).