气候变化领域集成服务门户(CCPortal): Structural insights into membrane remodeling by SNX1

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DOI	10.1073/pnas.2022614118
	Structural insights into membrane remodeling by SNX1
	Zhang Y.; Pang X.; Li J.; Xu J.; Hsu V.W.; Sun F.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	The sorting nexin (SNX) family of proteins deform the membrane to generate transport carriers in endosomal pathways. Here, we elucidate how a prototypic member, SNX1, acts in this process. Performing cryoelectron microscopy, we find that SNX1 assembles into a protein lattice that consists of helical rows of SNX1 dimers wrapped around tubular membranes in a crosslinked fashion. We also visualize the details of this structure, which provides a molecular understanding of how various parts of SNX1 contribute to its ability to deform the membrane. Moreover, we have compared the SNX1 structure with a previously elucidated structure of an endosomal coat complex formed by retromer coupled to a SNX, which reveals how the molecular organization of the SNX in this coat complex is affected by retromer. The comparison also suggests insight into intermediary stages of assembly that results in the formation of the retromer-SNX coat complex on the membrane. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Coat complex; Cryoelectron microscopy; Helical reconstruction; Membrane deformation; SNX1
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180339
作者单位	National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; Division of Rheumatology, Inflammation and Immunity, Brigham and Women’s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, United States; School of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China
推荐引用方式 GB/T 7714	Zhang Y.,Pang X.,Li J.,et al. Structural insights into membrane remodeling by SNX1[J],2021,118(10).
APA	Zhang Y.,Pang X.,Li J.,Xu J.,Hsu V.W.,&Sun F..(2021).Structural insights into membrane remodeling by SNX1.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Zhang Y.,et al."Structural insights into membrane remodeling by SNX1".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).