气候变化领域集成服务门户(CCPortal): Phytophthora sojae effector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection

CCPortal

DOI	10.1073/pnas.2018312118
	Phytophthora sojae effector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection
	Lin Y.; Hu Q.; Zhou J.; Yin W.; Yao D.; Shao Y.; Zhao Y.; Guo B.; Xia Y.; Chen Q.; Wang Y.; Ye W.; Xie Q.; Tyler B.M.; Xing W.; Wang Y.
发表日期	2021
ISSN	00278424
卷号	118 期号:10
英文摘要	Oomycete pathogens such as Phytophthora secrete a repertoire of effectors into host cells to manipulate host immunity and benefit infection. In this study, we found that an RxLR effector, Avr1d, promoted Phytophthora sojae infection in soybean hairy roots. Using a yeast two-hybrid screen, we identified the soybean E3 ubiquitin ligase GmPUB13 as a host target for Avr1d. By coimmunoprecipitation (Co-IP), gel infiltration, and isothermal titration calorimetry (ITC) assays, we confirmed that Avr1d interacts with GmPUB13 both in vivo and in vitro. Furthermore, we found that Avr1d inhibits the E3 ligase activity of GmPUB13. The crystal structure Avr1d in complex with GmPUB13 was solved and revealed that Avr1d occupies the binding site for E2 ubiquitin conjugating enzyme on GmPUB13. In line with this, Avr1d competed with E2 ubiquitin conjugating enzymes for GmPUB13 binding in vitro, thereby decreasing the E3 ligase activity of GmPUB13. Meanwhile, we found that inactivation of the ubiquitin ligase activity of GmPUB13 stabilized GmPUB13 by blocking GmPUB13 degradation. Silencing of GmPUB13 in soybean hairy roots decreased P. sojae infection, suggesting that GmPUB13 acts as a susceptibility factor. Altogether, this study highlights a virulence mechanism of Phytophthora effectors, by which Avr1d competes with E2 for GmPUB13 binding to repress the GmPUB13 E3 ligase activity and thereby stabilizing the susceptibility factor GmPUB13 to facilitate Phytophthora infection. This study unravels the structural basis for modulation of host targets by Phytophthora effectors and will be instrumental for boosting plant resistance breeding. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Crystal structure; Effector; Self-ubiquitination; Susceptibility factor; U-box
语种	英语
scopus关键词	GmPUB13 protein; ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2; ubiquitin protein ligase E3; unclassified drug; virulence factor; Article; binding site; chromatin immunoprecipitation; controlled study; crystal structure; downy mildew; enzyme activity; enzyme inactivation; enzyme stability; in vitro study; in vivo study; isothermal titration calorimetry; nonhuman; Phytophthora sojae; priority journal; protein analysis; protein binding; protein function; protein targeting; ubiquitination
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180335
作者单位	Department of Plant Pathology, Nanjing Agricultural University, Nanjing, 210095, China; The Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), Nanjing Agricultural University, Nanjing, 210095, China; The Key Laboratory of Plant Immunity, Nanjing Agricultural University, Nanjing, 210095, China; Shanghai Center for Plant Stress Biology, Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 200032, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Department of Plant Pathology, College of Plant Science and Technology, Key Lab of Crop Disease Monitoring and Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China; iHuman Institute, ShanghaiTech University, Shanghai, 201210, China; Ministry of Agriculture Key Lab of Pest Monitoring and Green Management, Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, 100193, China; State Key Labo...
推荐引用方式 GB/T 7714	Lin Y.,Hu Q.,Zhou J.,et al. Phytophthora sojae effector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection[J],2021,118(10).
APA	Lin Y..,Hu Q..,Zhou J..,Yin W..,Yao D..,...&Wang Y..(2021).Phytophthora sojae effector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection.Proceedings of the National Academy of Sciences of the United States of America,118(10).
MLA	Lin Y.,et al."Phytophthora sojae effector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection".Proceedings of the National Academy of Sciences of the United States of America 118.10(2021).