气候变化领域集成服务门户(CCPortal): Membrane bending by protein phase separation

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DOI	10.1073/pnas.2017435118
	Membrane bending by protein phase separation
	Yuan F.; Alimohamadi H.; Bakka B.; Trementozzi A.N.; Day K.J.; Fawzi N.L.; Rangamani P.; Stachowiak J.C.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	Membrane bending is a ubiquitous cellular process that is required for membrane traffic, cell motility, organelle biogenesis, and cell division. Proteins that bind to membranes using specific structural features, such as wedge-like amphipathic helices and crescentshaped scaffolds, are thought to be the primary drivers of membrane bending. However, many membrane-binding proteins have substantial regions of intrinsic disorder which lack a stable threedimensional structure. Interestingly, many of these disordered domains have recently been found to form networks stabilized by weak, multivalent contacts, leading to assembly of protein liquid phases on membrane surfaces. Here we ask how membraneassociated protein liquids impact membrane curvature. We find that protein phase separation on the surfaces of synthetic and cell-derived membrane vesicles creates a substantial compressive stress in the plane of the membrane. This stress drives the membrane to bend inward, creating protein-lined membrane tubules. A simple mechanical model of this process accurately predicts the experimentally measured relationship between the rigidity of the membrane and the diameter of the membrane tubules. Discovery of this mechanism, which may be relevant to a broad range of cellular protrusions, illustrates that membrane remodeling is not exclusive to structured scaffolds but can also be driven by the rapidly emerging class of liquid-like protein networks that assemble at membranes. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Membrane biophysics; Membrane curvature; Protein phase separation
语种	英语
scopus关键词	article; biophysics; human; membrane vesicle; phase separation; physical model; physiological stress; protein domain; rigidity
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180293
作者单位	Department of Biomedical Engineering, University of Texas at Austin, Austin, TX 78712, United States; Department of Mechanical and Aerospace Engineering, University of California San Diego, San Diego, CA 92093, United States; Department of Molecular Pharmacology, Physiology and Biotechnology, Brown University, Providence, RI 02912, United States; Institute for Cellular and Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, United States
推荐引用方式 GB/T 7714	Yuan F.,Alimohamadi H.,Bakka B.,et al. Membrane bending by protein phase separation[J],2021,118(11).
APA	Yuan F..,Alimohamadi H..,Bakka B..,Trementozzi A.N..,Day K.J..,...&Stachowiak J.C..(2021).Membrane bending by protein phase separation.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Yuan F.,et al."Membrane bending by protein phase separation".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).