气候变化领域集成服务门户(CCPortal): A unique C2 domain at the C terminus of Munc13 promotes synaptic vesicle priming

CCPortal

DOI	10.1073/pnas.2016276118
	A unique C2 domain at the C terminus of Munc13 promotes synaptic vesicle priming
	Padmanarayana M.; Liu H.; Michelassi F.; Li L.; Betensky D.; Dominguez M.J.; Sutton R.B.; Hu Z.; Dittman J.S.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	Neurotransmitter release during synaptic transmission comprises a tightly orchestrated sequence of molecular events, and Munc13-1 is a cornerstone of the fusion machinery. A forward genetic screen for defects in neurotransmitter release in Caenorhabditis elegans identified a mutation in the Munc13-1 ortholog UNC-13 that eliminated its unique and deeply conserved C-terminal module (referred to as HC2M) containing a Ca2+-insensitive C2 domain flanked by membrane-binding helices. The HC2M module could be functionally replaced in vivo by protein domains that localize to synaptic vesicles but not to the plasma membrane. HC2M is broadly conserved in other Unc13 family members and is required for efficient synaptic vesicle priming. We propose that the HC2M domain evolved as a vesicle/endosome adaptor and acquired synaptic vesicle specificity in the Unc13ABC protein family. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	C2; Caenorhabditis elegans; Munc13; Synaptic vesicle; Unc-13
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180272
作者单位	Department of Biochemistry, Weill Cornell Medical College, New York, NY 10065, United States; Queensland Brain Institute, Clem Jones Centre for Ageing Dementia Research, The University of Queensland, Brisbane, QLD 4072, Australia; Department of Cell Physiology and Molecular Biophysics, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, TX 79430, United States
推荐引用方式 GB/T 7714	Padmanarayana M.,Liu H.,Michelassi F.,et al. A unique C2 domain at the C terminus of Munc13 promotes synaptic vesicle priming[J],2021,118(11).
APA	Padmanarayana M..,Liu H..,Michelassi F..,Li L..,Betensky D..,...&Dittman J.S..(2021).A unique C2 domain at the C terminus of Munc13 promotes synaptic vesicle priming.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Padmanarayana M.,et al."A unique C2 domain at the C terminus of Munc13 promotes synaptic vesicle priming".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).