气候变化领域集成服务门户(CCPortal): A functional screen identifies transcriptional networks that regulate HIV-1 and HIV-2

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DOI	10.1073/pnas.2012835118
	A functional screen identifies transcriptional networks that regulate HIV-1 and HIV-2
	Pedro K.D.; Agosto L.M.; Sewell J.A.; Eberenz K.A.; He X.; Bass J.I.F.; Henderson A.J.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	The molecular networks involved in the regulation of HIV replication, transcription, and latency remain incompletely defined. To expand our understanding of these networks, we performed an unbiased high-throughput yeast one-hybrid screen, which identified 42 human transcription factors and 85 total protein-DNA interactions with HIV-1 and HIV-2 long terminal repeats. We investigated a subset of these transcription factors for transcriptional activity in cell-based models of infection. KLF2 and KLF3 repressed HIV-1 and HIV-2 transcription in CD4+T cells, whereas PLAGL1 activated transcription of HIV-2 through direct protein- DNA interactions. Using computational modeling with interacting proteins, we leveraged the results from our screen to identify putative pathways that define intrinsic transcriptional networks. Overall, we used a high-throughput functional screen, computational modeling, and biochemical assays to identify and confirm several candidate transcription factors and biochemical processes that influence HIV-1 and HIV-2 transcription and latency. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	HIV latency; HIV transcription; Yeast one-hybrid
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180269
作者单位	Department of Microbiology, Boston University School of Medicine, Boston, MA 02118, United States; Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, Boston, MA 02118, United States; Department of Biology Boston University, Boston, MA 02215, United States
推荐引用方式 GB/T 7714	Pedro K.D.,Agosto L.M.,Sewell J.A.,et al. A functional screen identifies transcriptional networks that regulate HIV-1 and HIV-2[J],2021,118(11).
APA	Pedro K.D..,Agosto L.M..,Sewell J.A..,Eberenz K.A..,He X..,...&Henderson A.J..(2021).A functional screen identifies transcriptional networks that regulate HIV-1 and HIV-2.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Pedro K.D.,et al."A functional screen identifies transcriptional networks that regulate HIV-1 and HIV-2".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).