气候变化领域集成服务门户(CCPortal): The diversity and evolution of microbial dissimilatory phosphite oxidation

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DOI	10.1073/pnas.2020024118
	The diversity and evolution of microbial dissimilatory phosphite oxidation
	Ewens S.D.; Gomberg A.F.S.; Barnum T.P.; Borton M.A.; Carlson H.K.; Wrighton K.C.; Coates J.D.
发表日期	2021
ISSN	00278424
卷号	118 期号:11
英文摘要	Phosphite is the most energetically favorable chemotrophic electron donor known, with a half-cell potential (Eo′) of -650 mV for the PO43-/PO33-couple. Since the discovery of microbial dissimilatory phosphite oxidation (DPO) in 2000, the environmental distribution, evolution, and diversity of DPO microorganisms (DPOMs) have remained enigmatic, as only two species have been identified. Here, metagenomic sequencing of phosphite-enriched microbial communities enabled the genome reconstruction and metabolic characterization of 21 additional DPOMs. These DPOMs spanned six classes of bacteria, including the Negativicutes, Desulfotomaculia, Synergistia, Syntrophia, Desulfobacteria, and Desulfomonilia-A. Comparing the DPO genes from the genomes of enriched organisms with over 17,000 publicly available metagenomes revealed the global existence of this metabolism in diverse anoxic environments, including wastewaters, sediments, and subsurface aquifers. Despite their newfound environmental and taxonomic diversity, metagenomic analyses suggested that the typical DPOM is a chemolithoautotroph that occupies lowoxygen environments and specializes in phosphite oxidation coupled to CO2reduction. Phylogenetic analyses indicated that the DPO genes form a highly conserved cluster that likely has ancient origins predating the split of monoderm and diderm bacteria. By coupling microbial cultivation strategies with metagenomics, these studies highlighted the unsampled metabolic versatility latent in microbial communities. We have uncovered the unexpected prevalence, diversity, biochemical specialization, and ancient origins of a unique metabolism central to the redox cycling of phosphorus, a primary nutrient on Earth. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	CO2fixation; Desulfotignum; Glycine reductive pathway; Phosphite; Phosphitivorax
语种	英语
scopus关键词	carbon dioxide; phosphite; phosphorus; aquifer; Article; chemolithoautotroph; chemotroph; controlled study; Desulfobacteria; Desulfomonilia; Desulfotomaculum; evolution; metagenome; metagenomics; microbial community; microbial diversity; microbial genome; Negativicutes; nonhuman; oxidation; oxidation reduction state; phylogeny; prevalence; priority journal; sediment; species identification; Syntrophia; wastewater
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/180254
作者单位	Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; Energy & Biosciences Institute, University of California, Berkeley, CA 94720, United States; Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO 80523, United States; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Ewens S.D.,Gomberg A.F.S.,Barnum T.P.,et al. The diversity and evolution of microbial dissimilatory phosphite oxidation[J],2021,118(11).
APA	Ewens S.D..,Gomberg A.F.S..,Barnum T.P..,Borton M.A..,Carlson H.K..,...&Coates J.D..(2021).The diversity and evolution of microbial dissimilatory phosphite oxidation.Proceedings of the National Academy of Sciences of the United States of America,118(11).
MLA	Ewens S.D.,et al."The diversity and evolution of microbial dissimilatory phosphite oxidation".Proceedings of the National Academy of Sciences of the United States of America 118.11(2021).