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DOI10.1073/pnas.2024705118
A localized adaptor protein performs distinct functions at the Caulobacter cell poles
Wang J.; Moerner W.E.; Shapiro L.
发表日期2021
ISSN00278424
卷号118期号:13
英文摘要Asymmetric cell division generates two daughter cells with distinct characteristics and fates. Positioning different regulatory and signaling proteins at the opposing ends of the predivisional cell produces molecularly distinct daughter cells. Here, we report a strategy deployed by the asymmetrically dividing bacterium Caulobacter crescentus where a regulatory protein is programmed to perform distinct functions at the opposing cell poles. We find that the CtrA proteolysis adaptor protein PopA assumes distinct oligomeric states at the two cell poles through asymmetrically distributed c-di-GMP: dimeric at the stalked pole and monomeric at the swarmer pole. Different polar organizing proteins at each cell pole recruit PopA where it interacts with and mediates the function of two molecular machines: the ClpXP degradation machinery at the stalked pole and the flagellar basal body at the swarmer pole. We discovered a binding partner of PopA at the swarmer cell pole that together with PopA regulates the length of the flagella filament. Our work demonstrates how a second messenger provides spatiotemporal cues to change the physical behavior of an effector protein, thereby facilitating asymmetry. © 2021 National Academy of Sciences. All rights reserved.
英文关键词Assembly; Asymmetry; Flagellar; Protein degradation; Second messenger; Single-particle tracking
语种英语
scopus关键词adaptor protein; cell protein; ClpXP protease; CtrA protein; cyclic GMP; dimer; endopeptidase ClpX; monomer; oligomer; PodJ protein; PopA protein; PopZ protein; regulator protein; unclassified drug; Article; asymmetric cell division; bacterial cell; bacterial flagellum; bimolecular fluorescence complementation assay; Caulobacter vibrioides; cell structure; cellular distribution; controlled study; dimerization; enzyme degradation; in vitro study; in vivo study; kinetosome; nonhuman; oligomerization; priority journal; protein binding; protein degradation; protein function; protein interaction assay; protein localization; protein protein interaction; regulatory mechanism; second messenger; single molecule imaging
来源期刊Proceedings of the National Academy of Sciences of the United States of America
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/180106
作者单位Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States; Chan Zuckerberg Biohub, San Francisco, CA 94158, United States
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Wang J.,Moerner W.E.,Shapiro L.. A localized adaptor protein performs distinct functions at the Caulobacter cell poles[J],2021,118(13).
APA Wang J.,Moerner W.E.,&Shapiro L..(2021).A localized adaptor protein performs distinct functions at the Caulobacter cell poles.Proceedings of the National Academy of Sciences of the United States of America,118(13).
MLA Wang J.,et al."A localized adaptor protein performs distinct functions at the Caulobacter cell poles".Proceedings of the National Academy of Sciences of the United States of America 118.13(2021).
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